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New vectors for simple and streamlined CRISPR-Cas9 genome editing in Saccharomyces cerevisiae.
Laughery MF, Hunter T, Brown A, Hoopes J, Ostbye T, Shumaker T, Wyrick JJ
Yeast. 2015 Dec;32(12):711-20. doi: 10.1002/yea.3098. Epub 2015 Sep 21.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
67638pML104Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation
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67639pML107Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation
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67640pT040URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette
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67641pJH001High copy Cas9 expression vector with LEU2 marker for yeast transformation
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