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pT040
(Plasmid #67640)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 67640 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCT3
  • Backbone size w/o insert (bp) 4749
  • Total vector size (bp) 5187
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Need to purify DNA from Dam- E.coli strain for efficient BclI cutting
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    single guide RNA expression cassette
  • gRNA/shRNA sequence
    target sequence into cassette is cloned using BclI-SwaI restriction enzymes
  • Species
    S. cerevisiae (budding yeast), Synthetic
  • Promoter pSNR52

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacII (not destroyed)
  • 3′ cloning site EagI (not destroyed)
  • 5′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT040 was a gift from John Wyrick (Addgene plasmid # 67640 ; http://n2t.net/addgene:67640 ; RRID:Addgene_67640)
  • For your References section:

    New vectors for simple and streamlined CRISPR-Cas9 genome editing in Saccharomyces cerevisiae. Laughery MF, Hunter T, Brown A, Hoopes J, Ostbye T, Shumaker T, Wyrick JJ. Yeast. 2015 Dec;32(12):711-20. doi: 10.1002/yea.3098. Epub 2015 Sep 21. 10.1002/yea.3098 PubMed 26305040