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Markus Ralser Lab Plasmids


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Title Authors Publication
pTREtight with high copy E. coli origin (unpublished)
Pyruvate kinase triggers a metabolic feedback loop that controls redox metabolism in respiring cells. Gruning NM, Rinnerthaler M, Bluemlein K, Mulleder M, Wamelink MM, Lehrach H, Jakobs C, Breitenbach M, Ralser M Cell Metab. 2011 Sep 7;14(3):415-27.
No evidence for a shift in pyruvate kinase PKM1 to PKM2 expression during tumorigenesis. Bluemlein K, Gruning NM, Feichtinger RG, Lehrach H, Kofler B, Ralser M Oncotarget. 2011 May;2(5):393-400.
A prototrophic deletion mutant collection for yeast metabolomics and systems biology. Mulleder M, Capuano F, Pir P, Christen S, Sauer U, Oliver SG, Ralser M Nat Biotechnol. 2012 Dec 7;30(12):1176-8. doi: 10.1038/nbt.2442.
Inhibition of triosephosphate isomerase by phosphoenolpyruvate in the feedback-regulation of glycolysis. Gruning NM, Du D, Keller MA, Luisi BF, Ralser M Open Biol. 2014 Mar 5;4(3):130232. doi: 10.1098/rsob.130232.
Interfering with glycolysis causes Sir2-dependent hyper-recombination of Saccharomyces cerevisiae plasmids. Ralser M, Zeidler U, Lehrach H PLoS One. 2009;4(4):e5376. doi: 10.1371/journal.pone.0005376. Epub 2009 Apr 24.
The pentose phosphate pathway is a metabolic redox sensor and regulates transcription during the antioxidant response. Kruger A, Gruning NM, Wamelink MM, Kerick M, Kirpy A, Parkhomchuk D, Bluemlein K, Schweiger MR, Soldatov A, Lehrach H, Jakobs C, Ralser M Antioxid Redox Signal. 2011 Jul 15;15(2):311-24. doi: 10.1089/ars.2010.3797. Epub 2011 May 19.
MitoLoc: A method for the simultaneous quantification of mitochondrial network morphology and membrane potential in single cells. Vowinckel J, Hartl J, Butler R, Ralser M Mitochondrion. 2015 Sep;24:77-86. doi: 10.1016/j.mito.2015.07.001. Epub 2015 Jul 13.
Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities (unpublished)
ID Kit Hidden Extra Search Info  
1000000085Yeast Prototrophy Kit Add to Cart
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
19407pTREtight2empty vectorMammalian Expression pTREtight with high copy E. coli origin (unpublished) pTREtight Add to Cart
19408pTREtight2-HA_GDP1GDP1Mammalian Expression pTREtight with high copy E. coli origin (unpublished) pTREtight2 KLLA0F09141g Add to Cart
34604p416GPD-PYK1PYK1 (Saccharomyces cerevisiae)Yeast ExpressionPyruvate kinase triggers a metabolic feedback loop that controls redox metabolism in respiring cells. Cell Metab. 2011 Sep 7;14(3):415-27. p416GPD Add to Cart
34605P413TEF-PYK1PYK1 (Saccharomyces cerevisiae)Yeast ExpressionPyruvate kinase triggers a metabolic feedback loop that controls redox metabolism in respiring cells. Cell Metab. 2011 Sep 7;14(3):415-27. P413TEF Add to Cart
34606p413TEF-PYK2PYK2 (Saccharomyces cerevisiae)Yeast ExpressionPyruvate kinase triggers a metabolic feedback loop that controls redox metabolism in respiring cells. Cell Metab. 2011 Sep 7;14(3):415-27. P413TEF PYK2 YOR347C Add to Cart
34607p413TEF-PKM1PKM1 (Homo sapiens)Yeast ExpressionNo evidence for a shift in pyruvate kinase PKM1 to PKM2 expression during tumorigenesis. Oncotarget. 2011 May;2(5):393-400. P413TEF Add to Cart
34608p413TEF-PKM2PKM2 (Homo sapiens)Yeast ExpressionNo evidence for a shift in pyruvate kinase PKM1 to PKM2 expression during tumorigenesis. Oncotarget. 2011 May;2(5):393-400. P413TEF PKM CTHBP, HEL-S-30, OIP3, PK3, PKM2, TCB, THBP1 Add to Cart
40276pHLUMLEU2 (Saccharomyces cerevisiae), URA3 (Saccharomyces cerevisiae), MET15/17 (Saccharomyces cerevisiae)Yeast ExpressionA prototrophic deletion mutant collection for yeast metabolomics and systems biology. Nat Biotechnol. 2012 Dec 7;30(12):1176-8. doi: 10.1038/nbt.2442. pRS313 LEU2 YCL018W URA3 YEL021W MET17 YLR303W, MET15, MET25 Add to Cart
50719p413GPD-hTPITriosephosphate isomerase 1 (Homo sapiens)Yeast ExpressionInhibition of triosephosphate isomerase by phosphoenolpyruvate in the feedback-regulation of glycolysis. Open Biol. 2014 Mar 5;4(3):130232. doi: 10.1098/rsob.130232. expression of human TPI1 in yeast p413GPD Add to Cart
50720p413GPD-hTPI Ile170ValTriosephosphate isomerase 1 (Homo sapiens)Yeast ExpressionInhibition of triosephosphate isomerase by phosphoenolpyruvate in the feedback-regulation of glycolysis. Open Biol. 2014 Mar 5;4(3):130232. doi: 10.1098/rsob.130232. expression of the human TPI Ile170Val allele in yeast p413GPD Add to Cart
50721p413GPD-hTPI Ile170ThrTriosephosphate isomerase 1 (Homo sapiens)Yeast ExpressionInhibition of triosephosphate isomerase by phosphoenolpyruvate in the feedback-regulation of glycolysis. Open Biol. 2014 Mar 5;4(3):130232. doi: 10.1098/rsob.130232. expression of the human TPI Ile170Thr allele in yeast p413GPD Add to Cart
50722p413GPD-hTPI Lys13ArgTriosephosphate isomerase 1 (Homo sapiens)Yeast ExpressionInhibition of triosephosphate isomerase by phosphoenolpyruvate in the feedback-regulation of glycolysis. Open Biol. 2014 Mar 5;4(3):130232. doi: 10.1098/rsob.130232. expression of the human TPI Lys13Arg allele in yeast p413GPD Add to Cart
50723pET20b- hTPITriosephosphate isomerase 1 (Homo sapiens)Bacterial ExpressionInhibition of triosephosphate isomerase by phosphoenolpyruvate in the feedback-regulation of glycolysis. Open Biol. 2014 Mar 5;4(3):130232. doi: 10.1098/rsob.130232. overexpression of human TPI1 in E.coli pET20b Add to Cart
50724pET20b- hTPI Ile170ValTriosephosphate isomerase 1 (Homo sapiens)Bacterial ExpressionInhibition of triosephosphate isomerase by phosphoenolpyruvate in the feedback-regulation of glycolysis. Open Biol. 2014 Mar 5;4(3):130232. doi: 10.1098/rsob.130232. overexpression of the human TPI1 Ile170Val allele in E.coli pET20b Add to Cart
50725pET20b- hTPI Ile170ThrTriosephosphate isomerase 1 (Homo sapiens)Bacterial ExpressionInhibition of triosephosphate isomerase by phosphoenolpyruvate in the feedback-regulation of glycolysis. Open Biol. 2014 Mar 5;4(3):130232. doi: 10.1098/rsob.130232. overexpression of the human TPI1 Ile170Thr allele in E.coli pET20b Add to Cart
50726pET20b- hTPI Lys13ArgTriosephosphate isomerase 1 (Homo sapiens)Bacterial ExpressionInhibition of triosephosphate isomerase by phosphoenolpyruvate in the feedback-regulation of glycolysis. Open Biol. 2014 Mar 5;4(3):130232. doi: 10.1098/rsob.130232. overexpression of the human TPI1 Lys13Arg allele in E.coli pET20b Add to Cart
51742p413TEF-Sir2Sir2 (Saccharomyces cerevisiae)Yeast ExpressionInterfering with glycolysis causes Sir2-dependent hyper-recombination of Saccharomyces cerevisiae plasmids. PLoS One. 2009;4(4):e5376. doi: 10.1371/journal.pone.0005376. Epub 2009 Apr 24. overexpression of Sir2 in S. cerevisiae, HIS3 marker p413TEF SIR2 YDL042C, MAR1 Add to Cart
51743p413TEF-Sir2-H364YSir2 (Saccharomyces cerevisiae)Yeast ExpressionInterfering with glycolysis causes Sir2-dependent hyper-recombination of Saccharomyces cerevisiae plasmids. PLoS One. 2009;4(4):e5376. doi: 10.1371/journal.pone.0005376. Epub 2009 Apr 24. overexpression of Sir2 in S. cerevisiae (H364Y mutant), HIS3 marker p413TEF SIR2 YDL042C, MAR1 Add to Cart
51744p413TEF-Sir2-P394LSir2 (Saccharomyces cerevisiae)Yeast ExpressionInterfering with glycolysis causes Sir2-dependent hyper-recombination of Saccharomyces cerevisiae plasmids. PLoS One. 2009;4(4):e5376. doi: 10.1371/journal.pone.0005376. Epub 2009 Apr 24. overexpression of Sir2 in S. cerevisiae (P394L mutant), HIS3 marker p413TEF SIR2 YDL042C, MAR1 Add to Cart
51745p413TEF-HST2HST2 (Saccharomyces cerevisiae)Yeast ExpressionInterfering with glycolysis causes Sir2-dependent hyper-recombination of Saccharomyces cerevisiae plasmids. PLoS One. 2009;4(4):e5376. doi: 10.1371/journal.pone.0005376. Epub 2009 Apr 24. overexpression of HST2 in S. cerevisiae, HIS3 marker p413TEF HST2 YPL015C Add to Cart
51746p413TEF-SirT1SirT1 (Homo sapiens)Yeast ExpressionInterfering with glycolysis causes Sir2-dependent hyper-recombination of Saccharomyces cerevisiae plasmids. PLoS One. 2009;4(4):e5376. doi: 10.1371/journal.pone.0005376. Epub 2009 Apr 24. overexpression of human SirT1 in S. cerevisiae, HIS3 marker p413TEF SIRT1 SIR2, SIR2L1, SIR2alpha Add to Cart
51747p413TEF-CARKLSHPK (Mus musculus)Yeast ExpressionThe pentose phosphate pathway is a metabolic redox sensor and regulates transcription during the antioxidant response. Antioxid Redox Signal. 2011 Jul 15;15(2):311-24. doi: 10.1089/ars.2010.3797. Epub 2011 May 19. overexpression of mouse Sedoheptulokinase (CARKL, SHPK) in yeast p413TEF Shpk RP23-214D6.1, 4930431K22Rik, AI194947, AW260459, Carkl Add to Cart
51748peGFPC2-CARKLSHPK (Mus musculus)Mammalian ExpressionThe pentose phosphate pathway is a metabolic redox sensor and regulates transcription during the antioxidant response. Antioxid Redox Signal. 2011 Jul 15;15(2):311-24. doi: 10.1089/ars.2010.3797. Epub 2011 May 19. expression of eGFP-fused mouse sedoheptulokinase (CARKL, SHPK) peGFP-C2 Shpk RP23-214D6.1, 4930431K22Rik, AI194947, AW260459, Carkl Add to Cart
58980pMitoLOCmCherry (Synthetic), GFP (Synthetic)Yeast ExpressionMitoLoc: A method for the simultaneous quantification of mitochondrial network morphology and membrane potential in single cells. Mitochondrion. 2015 Sep;24:77-86. doi: 10.1016/j.mito.2015.07.001. Epub 2015 Jul 13. Expression of preCOX4-mCherry & preSU9-GFP for colocalisation analysis of yeast mitochondrila pUG35 Add to Cart
64166pHLUM (version 2)Bacterial Expression, Yeast Expression Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities (unpublished) harbours marker genes HIS3, LEU2, URA3 and MET17 pRS313 Add to Cart
64167pHLUBacterial Expression, Yeast Expression Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities (unpublished) harbours marker genes HIS3, LEU2 and URA3 pHLUM (version 2) Add to Cart
64168pHLMBacterial Expression, Yeast Expression Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities (unpublished) harbours marker genes HIS3, LEU2 and MET17 pHLUM (version 2) Add to Cart
64169pLUMBacterial Expression, Yeast Expression Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities (unpublished) harbours marker genes LEU2, URA3 and MET17 pHLUM (version 2) Add to Cart
64170pHUMBacterial Expression, Yeast Expression Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities (unpublished) harbours marker genes HIS3, URA3 and MET17 pHLUM (version 2) Add to Cart
64171pHLBacterial Expression, Yeast Expression Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities (unpublished) harbours marker genes HIS3 and LEU2 pHLUM (version 2) Add to Cart
64172pHUBacterial Expression, Yeast Expression Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities (unpublished) harbours marker genes HIS3 and URA3 pHUM Add to Cart
64173pHMBacterial Expression, Yeast Expression Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities (unpublished) harbours marker genes HIS3 and MET17 pHLUM (version 2) Add to Cart
64174pLUBacterial Expression, Yeast Expression Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities (unpublished) harbours marker genes LEU2 and URA3 pHLUM (version 2) Add to Cart
64175pLMBacterial Expression, Yeast Expression Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities (unpublished) harbours marker genes LEU2 and MET17 pLUM Add to Cart
64176pUMBacterial Expression, Yeast Expression Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities (unpublished) harbours marker genes URA3 and MET17 pHUM Add to Cart
64177pLBacterial Expression, Yeast Expression Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities (unpublished) harbours marker gene LEU2 pHLUM (version 2) Add to Cart
64178pHBacterial Expression, Yeast Expression Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities (unpublished) harbours marker gene HIS3 pHLUM (version 2) Add to Cart
64179pMBacterial Expression, Yeast Expression Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities (unpublished) harbours marker gene MET17 pLUM Add to Cart
64180pUBacterial Expression, Yeast Expression Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities (unpublished) harbours marker gene URA3 pLU Add to Cart
64181pHLUKBacterial Expression, Yeast Expression Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities (unpublished) harbours marker genes HIS3, LEU2, URA3 and LYS2 pHLUM (version 2) Add to Cart
64182pHLKBacterial Expression, Yeast Expression Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities (unpublished) harbours marker genes HIS3, LEU2 and LYS2 pHLUK Add to Cart
64183pHUKBacterial Expression, Yeast Expression Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities (unpublished) harbours marker genes HIS3, URA3 and LYS2 pHLUK Add to Cart
64184pLUKBacterial Expression, Yeast Expression Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities (unpublished) harbours marker genes LEU2, URA3 and LYS2 pHLUK Add to Cart
64185pUKBacterial Expression, Yeast Expression Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities (unpublished) harbours marker genes URA3 and LYS2 pHUK Add to Cart
64186pLKBacterial Expression, Yeast Expression Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities (unpublished) harbours marker genes LEU2 and LYS2 pLUK Add to Cart
64187pHKBacterial Expression, Yeast Expression Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities (unpublished) harbours marker genes HIS3 and LYS2 pHLUK Add to Cart
64188pKBacterial Expression, Yeast Expression Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities (unpublished) harbours marker gene LYS2 pHK Add to Cart