165,214 results

1. pLKO.1 puro

   Plasmid

   #8453

   Purpose

   3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.

   Depositor

   Bob Weinberg

   Article

   Stewart et al RNA 2003 Apr;9(4):493-501.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUCmini-iCAP-PHP.eB

   Plasmid

   #103005

   Purpose

   non-standard AAV2 rep-AAV-PHP.eB cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   Synthetic construct isolate AAV-PHP.eB VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   p41

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-Sport6-CD63-pHluorin

   Plasmid

   #130901

   Purpose

   Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

   Depositor

   DM Pegtel

   Article

   Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

   Insert

   CD63-pHluorin (CD63 Aequorea victoria, Human)
   

   Tags

   pHluorin

   Expression

   Mammalian

   Mutation

   pHluorin inserted between Gln-36 and Leu-37 in ex…

   Promoter

   CMV

   Available Since

   Oct. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpyCatcher003-mi3

   Plasmid

   #159995

   Purpose

   Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   SpyCatcher003-mi3
   

   Tags

   C-Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV2/2

   Plasmid

   #232199

   Purpose

   AAV packaging plasmid expressing AAV2 Rep and capsid proteins

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   Rep2/Cap2
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Sept. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pLX313-Renilla luciferase

   Plasmid

   #118016

   Purpose

   Constitutive expression of Renilla luciferase

   Depositor

   William Hahn, David Root

   Article

   Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

   Insert

   Renilla luciferase
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSP1E3D1

   Plasmid

   #20066

   Purpose

   Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   Denisov et al J Biol Chem. 2007 Mar 9. 282(10):7066-76

   Insert

   MSP1E3D1
   

   Tags

   7-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1D1; contains repeats of …

   Available Since

   Jan. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3xHA-TurboID-NLS_pCDNA3

   Plasmid

   #107171

   Purpose

   expresses 3xHA-tagged TurboID in the mammalian nucleus

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Expression

   Mammalian

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. p70a-T7RNAP

   Plasmid

   #105683

   Purpose

   Expresses T7 polymerase in cell-free TXTL.

   Depositor

   Chase Beisel

   Article

   Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

   Insert

   T7 polymerase
   

   Expression

   Bacterial

   Promoter

   OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSM-mStayGold

    Plasmid

    #234317

    Purpose

    codon-optimized mStayGold with synthetic introns for the expression in C. elegans

    Depositor

    Kota Mizumoto

    Article

    Ko et al MicroPubl Biol. 2025 Jan 18;2025:10.1791

    Insert

    C. elegans codon optimized mStayGOld
    

    Tags

    mStayGold

    Expression

    Worm

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-PE6c

    Plasmid

    #207853

    Purpose

    Mammalian expression of PE6c prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6c
    

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-S33Y Beta-catenin

    Plasmid

    #19286

    Purpose

    Mammalian expression of human beta-catenin with Flag tag and S33Y mutation

    Depositor

    Eric Fearon

    Article

    Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

    Insert

    beta-catenin (CTNNB1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S33Y (presumptive GSK3β phosphorylation site)

    Available Since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLifeact-mScarlet3-H (mYongHong)

    Plasmid

    #239415

    Purpose

    Expresses Lifeact-mScarlet3-H (aka mYongHong) in mammalian cells

    Depositor

    Zhifei Fu

    Article

    Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

    Insert

    Lifeact-mScarlet3-H
    

    Expression

    Mammalian

    Mutation

    M163H in mScarlet3

    Promoter

    CMV

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. PiggyBac PlayBack

    Plasmid

    #203312

    Purpose

    To all for PiggyBac gene insertion into Genome

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-puro

    Plasmid

    #39481

    Purpose

    Tetracycline inducible 3rd generation lentiviral vector

    Depositor

    Ie-Ming Shih

    Article

    Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5 and 6XHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-PE6a

    Plasmid

    #207851

    Purpose

    Mammalian expression of PE6a prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6a
    

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Ec48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-T7pol

    Plasmid

    #59926

    Purpose

    Expression vector for T7 RNA polymerase gene

    Depositor

    Ian Wickersham

    Insert

    T7 RNA polymerase
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-Cre

    Plasmid

    #13775

    Purpose

    Mammalian expression of Cre recombinase from the broadly active CAG promoter/enhancer

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Cre
    

    Use

    Cre/Lox

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. human beta-catenin pcDNA3

    Plasmid

    #16828

    Purpose

    Mammalian expression of human beta-catenin with Flag tag

    Depositor

    Eric Fearon

    Article

    Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

    Insert

    beta-catenin (CTNNB1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiCas9-EGFP

    Plasmid

    #63592

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang, Phil Sharp

    Article

    Chen et al Cell. 2015 Mar 12;160(6):1246-60. doi: 1

    Inserts

    Cas9
    EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and P2A

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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