165,224 results

1. pAAV-U6-sgRNA-CMV-GFP

   Plasmid

   #85451

   Purpose

   Express sgRNA in mammalian cells

   Depositor

   Hetian Lei

   Article

   Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

   Insert

   pCMV-EGFP
   

   Use

   AAV

   Promoter

   pCMV-EGFP

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. EcMscL

   Plasmid

   #92418

   Purpose

   E. coli Mechanosensitive Channel of Large Conductance

   Depositor

   Douglas Rees

   Insert

   MscL (mscL E. coli)
   

   Tags

   6x Histidine Tag and Thrombin Cleavage Site betwe…

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-N-Flag-NLRP3

   Plasmid

   #75127

   Purpose

   Expresses N-terminal Flag-tagged NLRP3 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET3a-hUBA1

   Plasmid

   #63571

   Purpose

   Expression of human UBA1 tagged with His

   Depositor

   Titia Sixma

   Article

   Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

   Insert

   UBA1 (UBA1 Human)
   

   Expression

   Bacterial

   Mutation

   Isoform 2 (1018 amino acids)

   Promoter

   T7

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-miniTurbo-NLS-Blast

   Plasmid

   #232347

   Purpose

   Lentiviral control vector expressing miniTurboNLS without dCas9

   Depositor

   Ali Shilatifard

   Article

   Cenik et al Mol Cell. 2024 Dec 19;84(24):4929-4944.e

   Insert

   miniTurbo
   

   Use

   Lentiviral

   Tags

   3xHA and 3xSV40 NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLJM1-EGFP

   Plasmid

   #19319

   Purpose

   3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. ADORA3-Tango

   Plasmid

   #66212

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   ADORA3 (ADORA3 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. p53-GFP

   Plasmid

   #11770

   Depositor

   Geoff Wahl

   Insert

   p53 (TP53 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   72R variant of p53

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRRLsin-SV40 T antigen-IRES-mCherry

   Plasmid

   #58993

   Purpose

   lentiviral expression of SV40 large T antigen and mCherry red fluorescent reporter

   Depositor

   Snorri Thorgeirsson

   Article

   Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31

   Inserts

   CMV enhancer-chicken beta actin promoter
   SV40 large T antigen
   IRES-mCherry red fluorescent protein
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   EcoRV site in MCS converted to BamHI site

   Promoter

   CMV enhancer-chicken beta actin

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-ATF6

    Plasmid

    #32955

    Purpose

    For visualization of GFP-ATF6 in mammalian cells.

    Depositor

    Ron Prywes

    Article

    Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

    Insert

    ATF6 (ATF6 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    M67L and M313I

    Promoter

    CMV

    Available Since

    Nov. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV.CBA.eGFP.2A.P301L-Tau

    Plasmid

    #140425

    Purpose

    AAV.CBA.eGFP.2A.P301L-Tau propagation reporter P301L-2N4R-hTau

    Depositor

    Bradley Hyman

    Article

    Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

    Insert

    eGFP-2A-Tau(P301L) (MAPT Synthetic, Human)
    

    Use

    AAV

    Tags

    eGFP

    Mutation

    changed Proline 301 to Leucine

    Promoter

    CBA

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_AR-RE_MLPmin_BC1011-luc2

    Plasmid

    #227106

    Purpose

    Androgen receptor response element for luciferase and barcode assays; barcode BC1011

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    8x clustered AR response element linked to MLP minimal promoter driving barcode 1011 and a luciferase reporter gene
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. EF1a-Cas9-2XNES-2ERT2

    Plasmid

    #120551

    Purpose

    Express Cas9, 2xNES and 2 tandem ERT2 in mammalian cells

    Depositor

    Yu Wang

    Article

    Zhao et al Mol Ther Nucleic Acids. 2018 Dec 7;13:20

    Insert

    Cas9, NES and ERT2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. BAZ2AA-c002

    Plasmid

    #53623

    Purpose

    bacterial expression plasmid

    Depositor

    Nicola Burgess-Brown

    Insert

    BAZ2AA
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    Bacterial expression for structure determination;…

    Promoter

    T7

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pRK5_mEGFP-YAP-MAMLD1

    Plasmid

    #237675

    Purpose

    For overexpression of mEGFP-YAP-MAMLD1

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    YAP-MAMLD1 (YAP1, MAMLD1 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRK5_mEGFP-YAP-MAML2

    Plasmid

    #237674

    Purpose

    For overexpression of mEGFP-YAP-MAML2

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    YAP-MAML2 (YAP1, MAML2 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe-Kras G12D

    Plasmid

    #58902

    Purpose

    Expresses human Kras with a G12D Mutation

    Depositor

    Channing Der

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G12D mutation

    Available Since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. LV-AR

    Plasmid

    #213819

    Purpose

    Lenti-viral expression of human androgen receptor wild-type (WT).

    Depositor

    Steven Balk

    Article

    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

    Insert

    AR (human androgen receptor) (AR Human)
    

    Use

    Lentiviral

    Tags

    Flag (N terminal on backbone)

    Expression

    Mammalian

    Mutation

    No, it is AR wild-type.

    Promoter

    EF-1a

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. tFucci(CA)5

    Plasmid

    #153521

    Purpose

    New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(CA)5.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

    Insert

    tFucci(CA)5.
    

    Tags

    AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

    Expression

    Bacterial and Mammalian

    Promoter

    CAG

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-HA

    Plasmid

    #128034

    Purpose

    Mammalian expression vector with N terminal HA tag

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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