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pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) Citations (6)

Originally described in: Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Walton RT, Christie KA, Whittaker MN, Kleinstiver BP Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.
PubMed Journal

Articles Citing pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

STAT1 gain-of-function heterozygous cell models reveal diverse interferon-signature gene transcriptional responses. Scott O, Lindsay K, Erwood S, Mollica A, Roifman CM, Cohn RD, Ivakine EA. NPJ Genom Med. 2021 May 14;6(1):34. doi: 10.1038/s41525-021-00196-7. PubMed
Mutation-specific reporter for optimization and enrichment of prime editing. Schene IF, Joore IP, Baijens JHL, Stevelink R, Kok G, Shehata S, Ilcken EF, Nieuwenhuis ECM, Bolhuis DP, van Rees RCM, Spelier SA, van der Doef HPJ, Beekman JM, Houwen RHJ, Nieuwenhuis EES, Fuchs SA. Nat Commun. 2022 Mar 1;13(1):1028. doi: 10.1038/s41467-022-28656-3. PubMed

Associated Plasmids

Can SpRY recognize any PAM in human cells? Ye J, Xi H, Chen Y, Chen Q, Lu X, Lv J, Chen Y, Gu F, Zhao J. J Zhejiang Univ Sci B. 2022 May 15;23(5):382-391. doi: 10.1631/jzus.B2100710. PubMed
PAM-flexible dual base editor-mediated random mutagenesis and self-activation strategies to improve CRISPRa potency. Lau CH, Huang S, Lam RHW, Tin C. Mol Ther Methods Clin Dev. 2022 May 29;26:26-37. doi: 10.1016/j.omtm.2022.05.005. eCollection 2022 Sep 8. PubMed
Optimization of base editors for the functional correction of SMN2 as a treatment for spinal muscular atrophy. Alves CRR, Ha LL, Yaworski R, Sutton ER, Lazzarotto CR, Christie KA, Reilly A, Beauvais A, Doll RM, de la Cruz D, Maguire CA, Swoboda KJ, Tsai SQ, Kothary R, Kleinstiver BP. Nat Biomed Eng. 2023 Dec 6. doi: 10.1038/s41551-023-01132-z. PubMed

Associated Plasmids

Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathy. Grosch M, Schraft L, Chan A, Kuchenhoff L, Rapti K, Ferreira AM, Kornienko J, Li S, Radke MH, Kramer C, Clauder-Munster S, Perlas E, Backs J, Gotthardt M, Dieterich C, van den Hoogenhof MMG, Grimm D, Steinmetz LM. Nat Commun. 2023 Jun 22;14(1):3714. doi: 10.1038/s41467-023-39352-1. PubMed

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