Mutation-specific reporter for optimization and enrichment of prime editing.
Schene IF, Joore IP, Baijens JHL, Stevelink R, Kok G, Shehata S, Ilcken EF, Nieuwenhuis ECM, Bolhuis DP, van Rees RCM, Spelier SA, van der Doef HPJ, Beekman JM, Houwen RHJ, Nieuwenhuis EES, Fuchs SA
Nat Commun. 2022 Mar 1;13(1):1028. doi: 10.1038/s41467-022-28656-3. PubMed Article
Schene IF, Joore IP, Baijens JHL, Stevelink R, Kok G, Shehata S, Ilcken EF, Nieuwenhuis ECM, Bolhuis DP, van Rees RCM, Spelier SA, van der Doef HPJ, Beekman JM, Houwen RHJ, Nieuwenhuis EES, Fuchs SA
Nat Commun. 2022 Mar 1;13(1):1028. doi: 10.1038/s41467-022-28656-3. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 185476 | fluoPEER-HEK3_5bp-del | Fluorescent reporter for a 5 basepair deletion at the HEK3 locus. |
| 185477 | fluoPEER-GFP>BFP | Fluorescent reporter for a Y66H mutation to convert a GFP to BFP. |
| 185478 | pegRNA-HEK3_del1-5-NGG | pegRNA plasmid in order to make a 5 basepair deletion at the HEK3 locus, uses an NGG-PAM site. |
| 185480 | pegRNA-GFP>BFP_Y66H-NGG | pegRNA plasmid in order to make a Y66H (TAC>CAT) conversion in the GFP gene, creating a BFP gene. |