166,778 results

1. pCS2FA CO Tol2 TPase

   Plasmid

   #133032

   Purpose

   Expression vector for production of codon optimized Tol2 transposase

   Depositor

   Charles Nathan Hancock

   Article

   Mackey et al MicroPubl Biol. 2020 Jun 11;2020:10.1791

   Insert

   Codon optimized Tol2 Transposase
   

   Use

   In vitro expression

   Mutation

   Codon Optimized

   Available Since

   June 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221_SLC3A2

   Plasmid

   #132213

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC3A2 (SLC3A2 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pLenti_EF1a_Flag_EGFP

   Plasmid

   #183117

   Purpose

   Expresses flag-tagged EGFP in mammalian cells

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. BPK1520

   Plasmid

   #65777

   Purpose

   Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   SpCas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACYC-Skp-TF

   Plasmid

   #83921

   Purpose

   Co-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.

   Depositor

   Karl Griswold

   Article

   Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

   Inserts

   Skp
   Trigger Factor
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICH41780

   Plasmid

   #48019

   Depositor

   Sylvestre Marillonnet

   Article

   Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Dec. 3, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pAAV-N-RAM-d2tTA-TRE-mKate2

   Plasmid

   #140275

   Purpose

   N-RAM, the Npas4-dependent reporter

   Depositor

   Yingxi Lin

   Article

   Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

   Insert

   mKate2
   

   Use

   AAV

   Promoter

   TRE

   Available Since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLJM1-EGFP-GRAM-W

   Plasmid

   #211704

   Purpose

   Lentiviral expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (pLJM1-EGFP-GRAM1b G187W)

   Depositor

   Yasunori Saheki

   Article

   Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

   Insert

   EGFP-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Changed Glycine 187 to Tryptophan

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. CD8a

   Plasmid

   #86050

   Purpose

   A mammalian expression plasmid encoding CD8a without tag.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   CD8a (CD8A Human)
   

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEMG-GRIS

    Plasmid

    #225134

    Purpose

    For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Gm.

    Depositor

    Lars Blank

    Article

    Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Oct. 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. Integrin Beta1-mPA-GFP

    Plasmid

    #186302

    Purpose

    Expresses mPA-GFP tagged Integrin beta-1 in mammalian cells on a CMV promoter

    Depositor

    Pakorn Kanchanawong

    Article

    Jain et al Nat Protoc. 2022 Jul 27. pii: 10.1038/s4

    Insert

    Integrin Beta 1 (ITGB1 Human)
    

    Tags

    mPA-GFP

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLI50

    Plasmid

    #13573

    Depositor

    Chia Lee

    Article

    Lee et al Gene. 1991 Jul 15. 103(1):101-5.

    Type

    Empty backbone

    Use

    S. aureus-e. coli shuttle vector

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti6.2_TagBFP

    Plasmid

    #113724

    Purpose

    Expresses the fluorescent protein TagBFP in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Insert

    TagBFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. T7-VEE-mCherry

    Plasmid

    #193355

    Purpose

    Self-replicating RNA vector expressing mCherry fluorescent protein + PuroR

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    mCherry
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. EF1α-eGFP-Z1

    Plasmid

    #135612

    Purpose

    Enhanced green fluorescent protein expression for mammalian cells under EF1a-HTLV promoter in minimal backbone plasmid.

    Depositor

    Jordan Green

    Insert

    eGFP
    

    Expression

    Mammalian

    Promoter

    EF1a-HTLV

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST-CMV-polysite

    Plasmid

    #122843

    Purpose

    Gateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRX-IP-TMEM192-mKeima

    Plasmid

    #215358

    Purpose

    Probe to evaluate lysophagic activity

    Depositor

    Tamotsu Yoshimori

    Article

    Shima et al J Cell Biol. 2023 Dec 4;222(12):e2022040

    Insert

    transmembrane protein 192 (TMEM192 Human)
    

    Use

    Retroviral

    Tags

    mKeima

    Expression

    Mammalian

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFH2.2_mCherry-MS2x12

    Plasmid

    #205521

    Purpose

    Express mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRDA_118

    Plasmid

    #133459

    Purpose

    U6 promoter expresses customizable Spyo-guide; EF1a promoter provides puromycin resistance. This vector is a derivative of the lentiGuide vector, with minor modifications to the tracrR

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    control guide
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

    Plasmid

    #185911

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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