161,202 results

1. pTCF158 Human beta-2 microglobulin

   Plasmid

   #180457

   Purpose

   E. coli expression of class I MHC light chain

   Depositor

   John Altman

   Insert

   Human beta-2 microglobulin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant))

   Plasmid

   #218508

   Purpose

   integrative plasmid enabling constitutive expression of a destabilized mNeongreen reporter in Streptococcus pyogenes, targets the attP site of phage T12 in several S. pyogenes strains via an integrase

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   mNeongreen
   

   Use

   Tags

   modified ssrA degradation tag

   Expression

   Bacterial

   Mutation

   deletion of MCS containing Plac_lacZa, introducti…

   Promoter

   P23

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine

   Plasmid

   #50463

   Purpose

   Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Insert

   hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMaCTag-Z13

   Plasmid

   #120056

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mCherry

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mCherry
   

   Use

   Pcr template

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF0741

   Plasmid

   #142216

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   JDP2 (JDP2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pet28a-His6-Keap1

   Plasmid

   #62454

   Purpose

   His6 tagged Keap1 for E.coli expression

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Keap1 (KEAP1 Human)
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-FLAG-SOX4

   Plasmid

   #110360

   Purpose

   Mammalian expression of FLAG-tagged SOX4

   Depositor

   Carlos Moreno

   Article

   Liu et al Cancer Res. 2006 Apr 15;66(8):4011-9. do

   Insert

   SOX4 (SOX4 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   lacking 5' UTR

   Promoter

   Available since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. VHL-pGex2TK

   Plasmid

   #20790

   Depositor

   William Kaelin

   Article

   Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

   Insert

   VHL (VHL Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   VHL wt cDNA, no mutations

   Promoter

   Available since

   April 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVE13300

   Plasmid

   #137715

   Purpose

   Expresses impLbCas12a in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

   Insert

   impLbCas12a
   

   Use

   Tags

   3xHA, NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL0_9 [pUB110 ORI]

    Plasmid

    #198924

    Purpose

    Level 0 part

    Depositor

    Nili Ostrov, Charlie Gilbert, Cultivarium Tools

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pUB110 ORI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. AiP1888 - pAAV-AiE2575m-minBG-SYFP2-WPRE-BGHpA

    Plasmid

    #214570

    Purpose

    AiE2575m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Bosiljka Tasic, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBABEpuro-CRAF

    Plasmid

    #51124

    Purpose

    mammalian expression of wt CRAF

    Depositor

    Matthew Meyerson

    Insert

    CRAF (RAF1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-rab11 WT

    Plasmid

    #12674

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB11A (Homo sapiens) (RAB11A Human)
    

    Use

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. delCMV-mCherry-2x-Rhotekin-GBD

    Plasmid

    #214142

    Purpose

    Rho activity sensor

    Depositor

    Leif Dehmelt

    Article

    Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

    Insert

    3xRhotekin-GBD (Rtkn Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Only the GTPase binding domain (GBD) is included

    Promoter

    delCMV

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tn7 integration plasmid

    Plasmid

    #154134

    Purpose

    Suicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.

    Depositor

    Shimon Bershtein

    Article

    Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

    Inserts

    left and right end of Tn7 arm
    Spectinomycin resistant gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Spectinomycin resistance gene promoter and no

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC18-mini-Tn7T-Gm-lacZ

    Plasmid

    #63120

    Purpose

    mini-Tn7 lacZ transcriptional fusion vector

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 17, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pcDNA3-FLAG-Luciferase

    Plasmid

    #58792

    Purpose

    Express FLAG-tagged Luciferase in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    firefly Luciferase
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

18. hsgk1

    Plasmid

    #83432

    Purpose

    full length human sgk1 with Flag tag

    Depositor

    Christie Thomas

    Article

    Raikwar et al Am J Physiol Renal Physiol. 2008 Nov;295

    Insert

    human Sgk1 (SGK1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTCF156 HLA-A*02:01

    Plasmid

    #180456

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-A*02:01
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-HA-ER D538G

    Plasmid

    #49500

    Purpose

    Expresses HA-tagged ER D538G mutant in mammalian cells

    Depositor

    Sarat Chandarlapaty

    Article

    Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

    Insert

    ESR1 (ESR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Aspartic acid 538 to Glycine

    Promoter

    CMV

    Available since

    Dec. 12, 2013

    Availability

    Academic Institutions and Nonprofits only