Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is operating at a reduced capacity.

  • Pending order? We will email to confirm that your organization can accept shipments.
  • Conducting coronavirus or COVID-19 research? Email us at [email protected] with your order or deposit number so we can prioritize it.

Learn more about our current shipping status and COVID-19 resources.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pCMV6M-Pak1
(Plasmid #12209)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12209 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    JM109
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pak1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2000
  • Entrez Gene
    PAK1 (a.k.a. IDDMSSD, PAKalpha, alpha-PAK, p65-PAK)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hgh-pA-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Cloning sites: SalI-myc-BamHI-Pak1-EcoRI.

The PAK1 insert in this plasmid contains L516I and G401S mutations, or SNPs, that were present from the very beginning (~1995). The crystal structure of Pak1 uses this clone, so it is present there too. Depositing laboratory states that these mutations should not affect plasmid function, as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV6M-Pak1 was a gift from Jonathan Chernoff (Addgene plasmid # 12209 ; http://n2t.net/addgene:12209 ; RRID:Addgene_12209)
  • For your References section:

    Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells. Sells MA, Knaus UG, Bagrodia S, Ambrose DM, Bokoch GM, Chernoff J. Curr Biol. 1997 Mar 1. 7(3):202-10. 10.1016/S0960-9822(97)70091-5 PubMed 9395435