|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12209||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4900
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)2000
Entrez GenePAK1 (a.k.a. IDDMSSD, PAKalpha, alpha-PAK, p65-PAK)
- Promoter CMV
/ Fusion Protein
- Myc (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hgh-pA-R (Common Sequencing Primers)
Cloning sites: SalI-myc-BamHI-Pak1-EcoRI.
The PAK1 insert in this plasmid contains L516I and G401S mutations, or SNPs, that were present from the very beginning (~1995). The crystal structure of Pak1 uses this clone, so it is present there too. Depositing laboratory states that these mutations should not affect plasmid function, as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV6M-Pak1 was a gift from Jonathan Chernoff (Addgene plasmid # 12209 ; http://n2t.net/addgene:12209 ; RRID:Addgene_12209)
For your References section:Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells. Sells MA, Knaus UG, Bagrodia S, Ambrose DM, Bokoch GM, Chernoff J. Curr Biol. 1997 Mar 1. 7(3):202-10. 10.1016/S0960-9822(97)70091-5 PubMed 9395435