pCMV6M-Pak1
(Plasmid #12209)

• Depositing Lab: Jonathan Chernoff
• Publication: Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Backbone

• Vector backbone: pCMV6
• Backbone size w/o insert (bp): 4900
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): JM109
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pak1
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Entrez Gene: PAK1 (a.k.a. IDDMSSD, PAKalpha, alpha-PAK, p65-PAK)
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hgh-pA-R

Resource Information

• Articles Citing this Plasmid:
  • 16 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cloning sites: SalI-myc-BamHI-Pak1-EcoRI.

The PAK1 insert in this plasmid contains L516I and G401S mutations, or SNPs, that were present from the very beginning (~1995). The crystal structure of Pak1 uses this clone, so it is present there too. Depositing laboratory states that these mutations should not affect plasmid function, as described in the associated publication.