pCMV6M-Pak1 H83L H86L
(Plasmid #12211)

• Depositing Lab: Jonathan Chernoff
• Publication: Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Backbone

• Vector backbone: pCMV6
• Backbone size w/o insert (bp): 4900
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pak1
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Mutation: PBD (p21-binding domain) mutant: H83L, H86L (see depositor comment below)
• Entrez Gene: PAK1 (a.k.a. IDDMSSD, PAKalpha, alpha-PAK, p65-PAK)
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: TCGTTTAGTGAACCGTCAG
• 3′ sequencing primer: GGAACTTCCAAGGCCAGGA

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Created by 3-way ligation of PCR products: B/Sac-Sac/Eco-Eco/B.

Pak1 may contain mutations G401S and L516I. Depositor states that this should not affect plasmid function.