170,787 results
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Plasmid#202486PurposeExpresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutivelyDepositorInsertsulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Budding Yeast, Human)
ExpressionBacterialPromoterEM7Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pB-EF1a-CuO-KRAB-dCas9-BFP-CymR-Puro
Plasmid#248161PurposeExpresses KRAB fused to dCas9 and BFP upon cumate inductionDepositorInsertsKRAB-dCas9-HA-tagBFP
HA-CymR-T2A-puro
UseCRISPRTagsHA and tagBFPExpressionMammalianPromoterEf1aAvailable SinceJan. 27, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE
Plasmid#223175PurposeFor cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInserthU6_Rfxcas13d-DR1_BsmBI
UseLentiviralMutationDR1 A>TAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3HA-AR-LBD
Plasmid#89111Purposemammalian expression of human androgen receptor ligand binding domain amino acid residues 624-919 with N-terminal double HA tagDepositorInserthAR ligand binding domain (AR Human)
Tags2xHAExpressionMammalianMutationcontains Ligand Binding Domain (624-919)PromoterCMVAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-UTX
Plasmid#24168PurposeMammalian expression of human UTX with HA tagDepositorAvailable SinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
PX458 V3
Plasmid#226957PurposeCBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ERT2CreERT2
Plasmid#13777PurposeMammalian expression of conditionally active form of Cre recombinase (activated in response to tamoxifen)DepositorInsertERT2-Cre-ERT2 fusion protein
UseCre/LoxExpressionMammalianPromoterCAGAvailable SinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
Tet1-dCas9
Plasmid#136650PurposeTet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertTet1 (Tet1 Mouse)
UseCRISPRTags3XFLag-NLS-Tet1-dCas9-NLSExpressionMammalianPromoterCMVAvailable SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-JAK3
Plasmid#23944DepositorInsertJAK3 (JAK3 Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-AMBRA1-3xFLAG
Plasmid#172605PurposeExpresses 3xFLAG-tagged AMBRA1 in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PCNA
Plasmid#215117PurposeExpresses mCherry-PCNA in mammalian cells from a lentiviral vector.DepositorAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
JNK-KTR-mStrawberry reporter
Plasmid#158689PurposeThis plasmid is a JNK pathway reporter. It has CMV promoter driving the expression of JNK-KTR fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-LAMP1-EGFP-APEX2-V5
Plasmid#236402PurposeExpresses a fluorescent lysosome-localized APEX2DepositorInsertLAMP1-EGFP-APEX2-V5
UseRetroviralTagsAPEX2, EGFP, and V5ExpressionMammalianAvailable SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.iGluSnFR3.v857.IgK-NGR
Plasmid#196227PurposeFluorescent reporter for glutamate, third generation, variant 857 in NGR backbone. iGluSnFR3.v857.NGRDepositorInsertpAAV hSyn iGluSnFR3 v857.NGR
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and NGR TM DomainExpressionMammalianAvailable SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lyso-GA
Plasmid#209874PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the lysosomal membrane with human LAMP1DepositorInsertddGFP A
TagsLAMP1ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA hygro
Plasmid#104991PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
Mito-SNAP
Plasmid#206480PurposeEncodes a mitochondrially-targeted SNAP tag for mammalian cell expressionDepositorInsertMitochondrial targeting signal from COX8A fused to SNAP (derived from human MGMT)
TagsSNAPfExpressionMammalianPromoterCMVAvailable SinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP3653
Plasmid#222348PurposeExpression vector with PabstBR promoter, kanR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV: Flag-Akaluc_SV40:PuroR
Plasmid#183048PurposeExpresses FLAG-tagged AkaLuc (Far-Red Firefly luciferase) in mammalian cells.DepositorInsertAkaLuc
UseLentiviralTagsFLAGExpressionMammalianPromoterCMVAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep
Plasmid#92099PurposeDoyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.DepositorTypeEmpty backboneUseCRISPR and TALEN; ZfnExpressionMammalianPromoterTet-On 3G BidirectionalAvailable SinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only