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CYBL-SPI1-CEBPB-IRF5

Plasmid

#190719

Purpose

For microglia differentiation from iPSC

Depositor

Li Gan

Article

Drager et al Nat Neurosci. 2022 Aug 11. pii: 10.1038/

Insert

SPI1-CEBPB-IRF5

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Cepheid1b-ST-WPRE

Plasmid

#214967

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for brighter fluorescence

Depositor

Peng Zou

Article

Han et al Sci Adv. 2023 Nov 24;9(47):eadi4208. doi

Insert

Cepheid1b-ST

Use

AAV

Tags

Expression

Mutation

Promoter

human Synapsin

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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Stx5L-mCherry

Plasmid

#209844

Purpose

Expresses Stx5L-mCherry in mammalian cells

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

STX5 (STX5 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Wildtype Stx5 (with 55M)

Promoter

CMV

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLCK-mScarlet3_C1

Plasmid

#189771

Purpose

Construct for labelling plasma membranes in mammalian cells using LCK-mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

LCK-mScarlet3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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MSCVneo-HA-ER-Hoxb8

Plasmid

#222291

Purpose

The most commonly used plasmid for the generation of ER-Hoxb8 conditionally immortalized myeloid cell lines.

Depositor

David Sykes, Mark Kamps

Article

Wang et al Nat Methods. 2006 Apr;3(4):287-93.

Insert

ER-Hoxb8 (ESR1, Hoxb8 Mouse, Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

The estrogen receptor hormone binding domain cont…

Promoter

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSIV-D3psi/delta env/delta Vif/delta Vpr

Plasmid

#132928

Purpose

Production of SIV-VLPs containing Vpx protein

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

Gag, pol, and vpx

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-M-puro

Plasmid

#125839

Purpose

modifying MCS, replacing EcoRI to BamHI region with BamHI and EcoRI

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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2xMyc-tdMCP-SNAP (tdMCP standard)

Plasmid

#226291

Purpose

Lentiviral plasmid expressing a tdMCP-protein standard. Used in LABEL-seq abundance assays.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Inserts

2xMyc-tdMCP-SNAPtag
EGFP-IRES-BlastR

Use

Lentiviral

Tags

2xMyc-tdMCP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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GOSR2-eGFP

Plasmid

#209886

Purpose

Expresses human GOSR2 (GS27) fused to eGFP in mammalian cells

Depositor

Geert van den Bogaart

Insert

GOSR2 (GOSR2 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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GOSR1-mCherry

Plasmid

#209884

Purpose

Expresses human GOSR1 (GS28) fused to mChery in mammalian cells

Depositor

Geert van den Bogaart

Insert

GOSR1

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B1

Plasmid

#165082

Purpose

gRNA 1 of pair B for Stx5L-specific knockout

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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paavCAG-post-mGRASP-2A-dTomato

Plasmid

#34912

Purpose

post

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Insert

postsynaptic mGRASP

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

Nov. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_CoV2_D18

Plasmid

#170442

Purpose

Express SARS-CoV-2 WT spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 pseudotyped virus production.

Depositor

David Nemazee

Article

Rogers et al Science. 2020 Aug 21;369(6506):956-963.

Insert

SARS-CoV-2 spike D18 (S SARS-CoV-2, USA-WA1/2020 strain)

Use

Tags

NA

Expression

Mammalian

Mutation

18aa deletion in c-terminal tail

Promoter

CMV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLAMP1-mCherry

Plasmid

#45147

Depositor

Amy Palmer

Article

Van Engelenburg et al Nat Methods. 2010 Apr;7(4):325-30. doi:

Insert

LAMP1-mCherry (LAMP1 Human, Synthetic)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 4, 2013

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-GFP (PX461)

Plasmid

#48140

Purpose

Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Promoter

Cbh

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV SCH9

Plasmid

#212708

Purpose

A SCHEMA evolved AAV variant effective at targeting subventricular NSCs

Depositor

David Schaffer

Article

Ojala et al Mol Ther. 2018 Jan 3;26(1):304-319. doi:

Insert

AAV2 Rep SCH9 Cap

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pC1-HyPer-Red

Plasmid

#48249

Purpose

Genetically encoded hydrogen peroxide indicator with red fluorescence.

Depositor

Vsevolod Belousov

Article

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

HYPER-RED

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV R-CEPIA1er

Plasmid

#58216

Purpose

Red fluorescent indicator for calcium signaling in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

R-CEPIA1er

Use

Tags

myc

Expression

Mammalian

Mutation

R-GECO1 E300D M305L D329N F332I E336D N346K F361W…

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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sUPRa

Plasmid

#223242

Purpose

sUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levels

Depositor

Colin Akerman

Article

Chakrabarty et al Sci Rep. 2024 Jul 1;14(1):14990. doi: 10

Insert

sUPRa

Use

AAV

Tags

Expression

Mutation

Promoter

Custom

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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paavCAG-pre-mGRASP-mCerulean

Plasmid

#34910

Purpose

pre

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Insert

presynaptic mGRASP

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

Nov. 15, 2013

Availability

Academic Institutions and Nonprofits only

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