|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16643||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepCMV2-Tag 2A
- Backbone size w/o insert (bp) 4300
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Alt namePhosphatidylinositol 3-kinase
Alt namep110 alpha
SpeciesH. sapiens (human)
Entrez GenePIK3CA (a.k.a. CCM4, CLAPO, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha)
/ Fusion Protein
- Flag (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV / XmnI (destroyed during cloning)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
This plasmid has 4 mutations in the ORF, N114D, L178S, L401N, and L404P. These mutations do not alter the function of the encoded proteins.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PIK3CA-WT was a gift from Bert Vogelstein (Addgene plasmid # 16643 ; http://n2t.net/addgene:16643 ; RRID:Addgene_16643)
For your References section:High frequency of mutations of the PIK3CA gene in human cancers. Samuels Y, Wang Z, Bardelli A, Silliman N, Ptak J, Szabo S, Yan H, Gazdar A, Powell SM, Riggins GJ, Willson JK, Markowitz S, Kinzler KW, Vogelstein B, Velculescu VE. Science. 2004 Apr 23. 304(5670):554. 10.1126/science.1096502 PubMed 15016963