pCFJ150 - pDESTttTi5605[R4-R3]
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19329||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2354
Vector typeWorm Expression
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth instructionsccdB Survival T1 phage resistant cells. standard conditions.
Copy numberHigh Copy
SpeciesC. elegans (nematode)
Insert Size (bp)6777
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Invitrogen Gateway 3-fragment compatible destination vector. Contains C.Briggsae unc-119 rescue fragment. Also contains genomic regions flanking ttTi5605 Mos1 insertion to generate MosSCI inserts at this locus.
Please note that due to a bug in the Addgene map algorithm, the attR sites are depicted as attR1 and attR2, when in fact they are attR3 and attR4. Please refer to the author's map for an accurate depiction of these sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCFJ150 - pDESTttTi5605[R4-R3] was a gift from Erik Jorgensen (Addgene plasmid # 19329 ; http://n2t.net/addgene:19329 ; RRID:Addgene_19329)
For your References section:Single-copy insertion of transgenes in Caenorhabditis elegans. Christian Frøkjær-Jensen, M Wayne Davis, Christopher E Hopkins, Blake J Newman, Jason M Thumme, Søren-Peter Olesen, Morten Grunnet & Erik M Jorgensen. 10.1038/ng.248 PubMed 18953339