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pCFJ150 - pDESTttTi5605[R4-R3]
(Plasmid #19329)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 19329 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Unknown
  • Growth instructions
    ccdB Survival T1 phage resistant cells. standard conditions.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pDESTttTi5605[R4-R3]
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    6777

Cloning Information

Resource Information

Depositor Comments

Invitrogen Gateway 3-fragment compatible destination vector. Contains C.Briggsae unc-119 rescue fragment. Also contains genomic regions flanking ttTi5605 Mos1 insertion to generate MosSCI inserts at this locus.

Please note that due to a bug in the Addgene map algorithm, the attR sites are depicted as attR1 and attR2, when in fact they are attR3 and attR4. Please refer to the author's map for an accurate depiction of these sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCFJ150 - pDESTttTi5605[R4-R3] was a gift from Erik Jorgensen (Addgene plasmid # 19329 ; http://n2t.net/addgene:19329 ; RRID:Addgene_19329)
  • For your References section:

    Single-copy insertion of transgenes in Caenorhabditis elegans. Christian Frøkjær-Jensen, M Wayne Davis, Christopher E Hopkins, Blake J Newman, Jason M Thumme, Søren-Peter Olesen, Morten Grunnet & Erik M Jorgensen. 10.1038/ng.248 PubMed 18953339