pCFJ151 - ttTi5605_MCS
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19330||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2334
Vector typeWorm Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namettTi5605 targeting region
SpeciesC. elegans (nematode)
Insert Size (bp)5075
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Standard Multiple Cloning site vector to generate MosSCI inserts at ttTi5605 location. Contains C.Briggsae unc-119 rescue gene. Also contains genomic regions flanking the ttTi5605 Mos1 insertion. Use C.elegans strain EG4322 to generate insertions.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCFJ151 - ttTi5605_MCS was a gift from Erik Jorgensen (Addgene plasmid # 19330 ; http://n2t.net/addgene:19330 ; RRID:Addgene_19330)
For your References section:Single-copy insertion of transgenes in Caenorhabditis elegans. Christian Frøkjær-Jensen, M Wayne Davis, Christopher E Hopkins, Blake J Newman, Jason M Thumme, Søren-Peter Olesen, Morten Grunnet & Erik M Jorgensen. 10.1038/ng.248 PubMed 18953339