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pCDNA6A-EGFR WT
(Plasmid #42665)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 42665 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA6/myc-His A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EGFR
  • Alt name
    epidermal growth factor receptor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1200
  • Entrez Gene
    EGFR (a.k.a. ERBB, ERBB1, HER1, NISBD2, PIG61, mENA)
  • Tags / Fusion Proteins
    • Myc (C terminal on backbone)
    • 6xHis (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA6A-EGFR WT was a gift from Mien-Chie Hung (Addgene plasmid # 42665 ; http://n2t.net/addgene:42665 ; RRID:Addgene_42665)
  • For your References section:

    Characterization of a novel tripartite nuclear localization sequence in the EGFR family. Hsu SC, Hung MC. J Biol Chem. 2007 Apr 6;282(14):10432-40. Epub 2007 Feb 5. 10.1074/jbc.M610014200 PubMed 17283074