VCP/p97 cooperates with YOD1, UBXD1 and PLAA to drive clearance of ruptured lysosomes by autophagy.
Papadopoulos C, Kirchner P, Bug M, Grum D, Koerver L, Schulze N, Poehler R, Dressler A, Fengler S, Arhzaouy K, Lux V, Ehrmann M, Weihl CC, Meyer H
EMBO J. 2016 Oct 17. pii: e201695148. PubMed Article
Papadopoulos C, Kirchner P, Bug M, Grum D, Koerver L, Schulze N, Poehler R, Dressler A, Fengler S, Arhzaouy K, Lux V, Ehrmann M, Weihl CC, Meyer H
EMBO J. 2016 Oct 17. pii: e201695148. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 85662 | pmCherry-Gal3 | Fluorescent reporter for lysosomal damage |
| 85663 | mCherry-YOD1 | Expression of human YOD1 with N-terminal mCherry tag |
| 85664 | pEGFP-YOD1 | Expression of human YOD1 with N-terminal GFP tag |
| 85666 | pEGFP-YOD1-C160S | Expression of human YOD1 C160S mutant with N-terminal GFP tag |
| 85667 | pEGFP-YOD1-MutS2 | Expression of human YOD1 I292Q V295Q mutant with N-terminal GFP tag |
| 85668 | pEGFP-YOD1-MutS2-C160S | Expression of human YOD1 I292Q V295Q C160S mutant with N-terminal GFP tag |
| 85669 | pEGFP-PLAA | Expression of PLAA with N-terminal GFP tag |
| 85670 | pEGFP-p97 | Expression of p97 with N-terminal GFP tag |
| 85671 | pEGFP-VCPIP1 | Expression of VCPIP1 with N-terminal GFP tag |
| 85672 | pEGFP-p97-E578Q | Expression of human p97 E578Q mutant with N-terminal GFP tag |
| 86464 | UBXD1-GFP | Expression of human UBXD1 with C-terminal GFP tag |