pEGFP-VCPIP1
(Plasmid #85671)

• Purpose: Expression of VCPIP1 with N-terminal GFP tag
• Depositing Lab: Hemmo Meyer
• Publication: Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Backbone

• Vector backbone: pEGFP-C1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: VCPIP1
• Alt name: valosin containing protein (p97)/p47 complex interacting protein 1
• Alt name: DKFZp686G038, FLJ23132, KIAA1850, VCIP135
• Species: H. sapiens (human)
• GenBank ID: BC094799
• Entrez Gene: VCPIP1 (a.k.a. DUBA3, VCIP135)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: cloned from Flag-HA-VCPIP1 (#22592, W. Harper)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry