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eIF2a 2
(Plasmid #21808)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21808 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA.CD2
  • Backbone manufacturer
    Addgene plasmid 21810
  • Backbone size w/o insert (bp) 5832
  • Vector type
    Mammalian Expression
  • Selectable markers
    CD2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eIF2a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1683
  • Mutation
    S52A
  • Entrez Gene
    EIF2S1 (a.k.a. EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Addgene sequencing results show small insertion in the MCS, upstream of EcoRI site. ORF is not affected. This insert is one of a few discrepancies between Addgene's quality control sequence and the sequence provided by the Ron lab. These discrepancies should not affect the function of the plasmid, but they have changed some of the restriction sites in the MCS. Please proceed cautiously when doing diagnostic digests or subcloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    eIF2a 2 was a gift from David Ron (Addgene plasmid # 21808 ; http://n2t.net/addgene:21808 ; RRID:Addgene_21808)