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PERK 2: PERK.K618A.9E10.pCDNA
(Plasmid #21815)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21815 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Growth instructions
    We have found that the PERK cDNA confers instability (low replication ability) to plasmids. The plasmid will be provided in the STBL2 bacterial strain (gibco) which helps alleviate this problem. To recover the plasmid, stick a sterile toothpick into the stab and streak on an LB amp plate and grow at 30C. Pick an individual colony and grow in LB amp at 30C for the highest yield of plasmid.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PERK (K618A)
  • Alt name
    Eif2ak3 eukaryotic translation initiation factor 2 alpha kinase 3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3577
  • Mutation
    kinase dead K618A mutant
  • Entrez Gene
    Eif2ak3 (a.k.a. Pek, Perk)
  • Tag / Fusion Protein
    • Myc (9E10) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Mouse PERK, K618A, 9E10-tagged at C-terminus, mammalian expression vector pCDNAamp.

The K->A mutation introduces a unique Nru1 site at position 5070. An EcoR1-Nru1 digest thus readily distinguishes the wild-type and mutant plasmids

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PERK 2: PERK.K618A.9E10.pCDNA was a gift from David Ron (Addgene plasmid # 21815 ; http://n2t.net/addgene:21815 ; RRID:Addgene_21815)