PERK 2: PERK.K618A.9E10.pCDNA
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21815||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4756
Vector typeMammalian Expression
Growth in Bacteria
Growth instructionsWe have found that the PERK cDNA confers instability (low replication ability) to plasmids. The plasmid will be provided in the STBL2 bacterial strain (gibco) which helps alleviate this problem. To recover the plasmid, stick a sterile toothpick into the stab and streak on an LB amp plate and grow at 30C. Pick an individual colony and grow in LB amp at 30C for the highest yield of plasmid.
Copy numberLow Copy
Gene/Insert namePERK (K618A)
Alt nameEif2ak3 eukaryotic translation initiation factor 2 alpha kinase 3
SpeciesM. musculus (mouse)
Insert Size (bp)3577
Mutationkinase dead K618A mutant
Entrez GeneEif2ak3 (a.k.a. Pek, Perk)
/ Fusion Protein
- Myc (9E10) (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Mouse PERK, K618A, 9E10-tagged at C-terminus, mammalian expression vector pCDNAamp.
The K->A mutation introduces a unique Nru1 site at position 5070. An EcoR1-Nru1 digest thus readily distinguishes the wild-type and mutant plasmids
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PERK 2: PERK.K618A.9E10.pCDNA was a gift from David Ron (Addgene plasmid # 21815 ; http://n2t.net/addgene:21815 ; RRID:Addgene_21815)