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Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta signaling.
Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias MJ, Erdjument-Bromage H, Tempst P, Massague J
Mol Cell. 2009 Nov 13. 36(3):457-68.
PubMed Article

Plasmids from Article

ID Plasmid  
26996pCI Flag Smad3 LC WT
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26997pCS2 Flag Smad3 T179V
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26998pCS2 Flag Smad3 EPSM V179T
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26999pCI HA NEDD4 DD
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27000pCI HA NEDD4L
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27001pCI HA NEDD4L DD
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27002pCI HA NEDD4
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27003pCI HA NEDD4L S382A S468A
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27004pCS2 Flag Smad3 AAGY
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27005pCI Flag Smad3 NL* EPSM
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27006pCI Flag Smad3 NL EPSM
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27010pGEX6-Smad3 WT
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27011pGEX6-Smad3 T179V
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27013pGEX6-Smad3 AAGY
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27015pGEX6-Smad3 EPSM V179T
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27016pRS-puro shNEDD4L #1
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27017pRS-puro shNEDD4L #2
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27025pQCXIH-Flag Smad3 WT
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27026pQCXIH-Flag Smad3 EPSM
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27027pQCXIH-Flag Smad3 AAGY
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27028pQCXIH-HA NEDD4L
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27036pCI HA NEDD4L S382A
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27037pCI HA NEDD4L S468A
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27038pCI Flag Smad3 LC EPSM
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27039pCI Flag Smad3 LC AAGY
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27040pCI Flag Smad3 NL WT
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27041pCI Flag Smad3 NL AAGY
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27042pCI Flag Smad3 NL* WT
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27108pGEX6-Smad3 EPSM A204S
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27109pGEX6-Smad3 EPSM A208S
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27110pGEX6-Smad3 EPSM A213S
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27111pCS2 Flag Smad3 EPSM A204S
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27112pCS2 Flag Smad3 EPSM A208S
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27113pCS2 Flag Smad3 EPSM A213S
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27114pCS2 Flag Smad3 S204A
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27115pCS2 Flag Smad3 S208A
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27116pCS2 Flag Smad3 S213A
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27222pGEX6-Smad3 EPSM
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