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pGEX6-Smad3 EPSM A213S
(Plasmid #27110)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27110 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-6P
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smad3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Mutation
    T179V S204A S208A S213A; A213S
  • Entrez Gene
    SMAD3 (a.k.a. HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3)
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pGEX-5
  • 3′ sequencing primer pGEX-3
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

A213 was mutated back to the wild type residue in EPSM construct

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX6-Smad3 EPSM A213S was a gift from Joan Massague (Addgene plasmid # 27110 ; http://n2t.net/addgene:27110 ; RRID:Addgene_27110)
  • For your References section:

    Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta signaling. Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias MJ, Erdjument-Bromage H, Tempst P, Massague J. Mol Cell. 2009 Nov 13. 36(3):457-68. 10.1016/j.molcel.2009.09.043 PubMed 19917253