pCMV5 Flag-Smad2 EPSM
(Plasmid #14933)

• Depositing Lab: Joan Massague
• Publication: Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

Backbone

• Vector backbone: pCMV5
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Smad2 EPSM
• Alt name: Smad2
• Species: H. sapiens (human)
• Insert Size (bp): 1400
• Mutation: Erk/Pro-directed kinase site mutant (EPSM). T220V, S245A, S250A, S255A.
• Entrez Gene: SMAD2 (a.k.a. CHTD8, JV18, JV18-1, LDS6, MADH2, MADR2, hMAD-2, hSMAD2)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See article for more information.