pCMV5 Flag-Smad2 EPSM
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14933||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameSmad2 EPSM
SpeciesH. sapiens (human)
Insert Size (bp)1400
MutationErk/Pro-directed kinase site mutant (EPSM). T220V, S245A, S250A, S255A.
Entrez GeneSMAD2 (a.k.a. JV18, JV18-1, MADH2, MADR2, hMAD-2, hSMAD2)
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
See article for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV5 Flag-Smad2 EPSM was a gift from Joan Massague (Addgene plasmid # 14933 ; http://n2t.net/addgene:14933 ; RRID:Addgene_14933)
For your References section:A mechanism of repression of TGFbeta/ Smad signaling by oncogenic Ras. Kretzschmar M, Doody J, Timokhina I, Massague J. Genes Dev. 1999 Apr 1. 13(7):804-16. 10.1101/gad.13.7.804 PubMed 10197981
Map uploaded by the depositor.