pCMV5 Flag-Smad1 (4SP/AP+AAVA)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14955||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameSmad1 (4SP/AP+AAVA)
SpeciesH. sapiens (human)
Insert Size (bp)1400
MutationFour Serine to Alanine mutations in the linker region; three Serine to Alanine mutations in the C-terminal sequence SSVS
Entrez GeneSMAD1 (a.k.a. BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1)
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Terms and Licenses
See author's map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV5 Flag-Smad1 (4SP/AP+AAVA) was a gift from Joan Massague (Addgene plasmid # 14955 ; http://n2t.net/addgene:14955 ; RRID:Addgene_14955)
For your References section:The TGF-beta family mediator Smad1 is phosphorylated directly and activated functionally by the BMP receptor kinase. Kretzschmar M, Liu F, Hata A, Doody J, Massague J. Genes Dev. 1997 Apr 15. 11(8):984-95. 10.1101/gad.11.8.984 PubMed 9136927
Map uploaded by the depositor.