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CS2 Flag-Smad3 (EPSM)
(Plasmid #14963)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14963 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4100
  • Vector type
    xenopus/mammalian/avian/zebrafish

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smad3 (EPSM)
  • Alt name
    Smad3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Mutation
    T178V, S203A, S207A, S212A
  • Entrez Gene
    SMAD3 (a.k.a. HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer EBV rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

3SP and 1TP sites in the linker are mutated to alanine or valine (T-DV). See author's map for more information. Please note that the mutations correspond to residues 179, 204, 208, and 213 of the native Smad3 protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CS2 Flag-Smad3 (EPSM) was a gift from Joan Massague (Addgene plasmid # 14963 ; http://n2t.net/addgene:14963 ; RRID:Addgene_14963)
  • For your References section:

    A mechanism of repression of TGFbeta/ Smad signaling by oncogenic Ras. Kretzschmar M, Doody J, Timokhina I, Massague J. Genes Dev. 1999 Apr 1. 13(7):804-16. 10.1101/gad.13.7.804 PubMed 10197981