CS2 Flag-Smad3 (EPSM)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14963||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameSmad3 (EPSM)
SpeciesH. sapiens (human)
Insert Size (bp)1300
MutationT178V, S203A, S207A, S212A
Entrez GeneSMAD3 (a.k.a. HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3)
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer EBV rev (Common Sequencing Primers)
3SP and 1TP sites in the linker are mutated to alanine or valine (T-DV). See author's map for more information. Please note that the mutations correspond to residues 179, 204, 208, and 213 of the native Smad3 protein.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CS2 Flag-Smad3 (EPSM) was a gift from Joan Massague (Addgene plasmid # 14963 ; http://n2t.net/addgene:14963 ; RRID:Addgene_14963)
For your References section:A mechanism of repression of TGFbeta/ Smad signaling by oncogenic Ras. Kretzschmar M, Doody J, Timokhina I, Massague J. Genes Dev. 1999 Apr 1. 13(7):804-16. 10.1101/gad.13.7.804 PubMed 10197981
Map uploaded by the depositor.