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pET His6 TEV LIC cloning vector (1B) Citations (41)

Originally described in: LIC N-terminal fusion vectors for E. coli expression

Articles Citing pET His6 TEV LIC cloning vector (1B)

Mutagenesis of GATA motifs controlling the endoderm regulator elt-2 reveals distinct dominant and secondary cis-regulatory elements. Du L, Tracy S, Rifkin SA. Dev Biol. 2016 Apr 1;412(1):160-70. doi: 10.1016/j.ydbio.2016.02.013. Epub 2016 Feb 16. PubMed
Histone deacetylase 6 structure and molecular basis of catalysis and inhibition. Hai Y, Christianson DW. Nat Chem Biol. 2016 Sep;12(9):741-7. doi: 10.1038/nchembio.2134. Epub 2016 Jul 25. PubMed

Associated Plasmids

RNA Primer Extension Hinders DNA Synthesis by Escherichia coli Mutagenic DNA Polymerase IV. Tashjian TF, Lin I, Belt V, Cafarelli TM, Godoy VG. Front Microbiol. 2017 Mar 1;8:288. doi: 10.3389/fmicb.2017.00288. eCollection 2017. PubMed
Exploring the Influence of Domain Architecture on the Catalytic Function of Diterpene Synthases. Pemberton TA, Chen M, Harris GG, Chou WK, Duan L, Koksal M, Genshaft AS, Cane DE, Christianson DW. Biochemistry. 2017 Apr 11;56(14):2010-2023. doi: 10.1021/acs.biochem.7b00137. Epub 2017 Mar 31. PubMed
Structural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a. Swarts DC, van der Oost J, Jinek M. Mol Cell. 2017 Apr 20;66(2):221-233.e4. doi: 10.1016/j.molcel.2017.03.016. PubMed
Backbone and side chain resonance assignments for a structured domain within Atg32. Xia X, Pellegrini M, Ragusa MJ. Biomol NMR Assign. 2017 Oct;11(2):211-214. doi: 10.1007/s12104-017-9750-y. Epub 2017 Aug 1. PubMed
Structure and function of yeast Atg20, a sorting nexin that facilitates autophagy induction. Popelka H, Damasio A, Hinshaw JE, Klionsky DJ, Ragusa MJ. Proc Natl Acad Sci U S A. 2017 Nov 21;114(47):E10112-E10121. doi: 10.1073/pnas.1708367114. Epub 2017 Nov 7. PubMed
Characterizing a thermostable Cas9 for bacterial genome editing and silencing. Mougiakos I, Mohanraju P, Bosma EF, Vrouwe V, Finger Bou M, Naduthodi MIS, Gussak A, Brinkman RBL, van Kranenburg R, van der Oost J. Nat Commun. 2017 Nov 21;8(1):1647. doi: 10.1038/s41467-017-01591-4. PubMed

Associated Plasmids

Bacteriophage DNA glucosylation impairs target DNA binding by type I and II but not by type V CRISPR-Cas effector complexes. Vlot M, Houkes J, Lochs SJA, Swarts DC, Zheng P, Kunne T, Mohanraju P, Anders C, Jinek M, van der Oost J, Dickman MJ, Brouns SJJ. Nucleic Acids Res. 2018 Jan 25;46(2):873-885. doi: 10.1093/nar/gkx1264. PubMed
A pseudo-receiver domain in Atg32 is required for mitophagy. Xia X, Katzenell S, Reinhart EF, Bauer KM, Pellegrini M, Ragusa MJ. Autophagy. 2018;14(9):1620-1628. doi: 10.1080/15548627.2018.1472838. Epub 2018 Aug 1. PubMed
Pause sequences facilitate entry into long-lived paused states by reducing RNA polymerase transcription rates. Gabizon R, Lee A, Vahedian-Movahed H, Ebright RH, Bustamante CJ. Nat Commun. 2018 Jul 26;9(1):2930. doi: 10.1038/s41467-018-05344-9. PubMed
A Potent and Selective PARP11 Inhibitor Suggests Coupling between Cellular Localization and Catalytic Activity. Kirby IT, Kojic A, Arnold MR, Thorsell AG, Karlberg T, Vermehren-Schmaedick A, Sreenivasan R, Schultz C, Schuler H, Cohen MS. Cell Chem Biol. 2018 Dec 20;25(12):1547-1553.e12. doi: 10.1016/j.chembiol.2018.09.011. Epub 2018 Oct 18. PubMed
Development of novel antibodies for detection of mobile colistin-resistant bacteria contaminated in meats. He X, Mavrici D, Patfield S, Rubio FM. Sci Rep. 2018 Nov 13;8(1):16744. doi: 10.1038/s41598-018-34764-2. PubMed
CRISPR-Cas ribonucleoprotein mediated homology-directed repair for efficient targeted genome editing in microalgae Nannochloropsis oceanica IMET1. Naduthodi MIS, Mohanraju P, Sudfeld C, D'Adamo S, Barbosa MJ, van der Oost J. Biotechnol Biofuels. 2019 Mar 25;12:66. doi: 10.1186/s13068-019-1401-3. eCollection 2019. PubMed

Associated Plasmids

Non-equilibrium dynamics of a nascent polypeptide during translation suppress its misfolding. Alexander LM, Goldman DH, Wee LM, Bustamante C. Nat Commun. 2019 Jun 20;10(1):2709. doi: 10.1038/s41467-019-10647-6. PubMed
The structure of a highly-conserved picocyanobacterial protein reveals a Tudor domain with an RNA-binding function. Bauer KM, Dicovitsky R, Pellegrini M, Zhaxybayeva O, Ragusa MJ. J Biol Chem. 2019 Sep 27;294(39):14333-14344. doi: 10.1074/jbc.RA119.007938. Epub 2019 Aug 7. PubMed
A new class of disordered elements controls DNA replication through initiator self-assembly. Parker MW, Bell M, Mir M, Kao JA, Darzacq X, Botchan MR, Berger JM. Elife. 2019 Sep 27;8. pii: 48562. doi: 10.7554/eLife.48562. PubMed
A photoactivatable crosslinking system reveals protein interactions in the Toxoplasma gondii inner membrane complex. Choi CP, Moon AS, Back PS, Jami-Alahmadi Y, Vashisht AA, Wohlschlegel JA, Bradley PJ. PLoS Biol. 2019 Oct 4;17(10):e3000475. doi: 10.1371/journal.pbio.3000475. eCollection 2019 Oct. PubMed
The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis. Bersuker K, Hendricks JM, Li Z, Magtanong L, Ford B, Tang PH, Roberts MA, Tong B, Maimone TJ, Zoncu R, Bassik MC, Nomura DK, Dixon SJ, Olzmann JA. Nature. 2019 Nov;575(7784):688-692. doi: 10.1038/s41586-019-1705-2. Epub 2019 Oct 21. PubMed
Structural and Functional Characterization of Stx2k, a New Subtype of Shiga Toxin 2. Hughes AC, Zhang Y, Bai X, Xiong Y, Wang Y, Yang X, Xu Q, He X. Microorganisms. 2019 Dec 18;8(1). pii: microorganisms8010004. doi: 10.3390/microorganisms8010004. PubMed
Structure and Mechanism of a Cyclic Trinucleotide-Activated Bacterial Endonuclease Mediating Bacteriophage Immunity. Lau RK, Ye Q, Birkholz EA, Berg KR, Patel L, Mathews IT, Watrous JD, Ego K, Whiteley AT, Lowey B, Mekalanos JJ, Kranzusch PJ, Jain M, Pogliano J, Corbett KD. Mol Cell. 2020 Feb 20;77(4):723-733.e6. doi: 10.1016/j.molcel.2019.12.010. Epub 2020 Jan 10. PubMed
A microparticulate based formulation to protect therapeutic enzymes from proteolytic digestion: phenylalanine ammonia lyase as case study. Pereira de Sousa I, Gourmel C, Berkovska O, Burger M, Leroux JC. Sci Rep. 2020 Feb 27;10(1):3651. doi: 10.1038/s41598-020-60463-y. PubMed
Guide-free Cas9 from pathogenic Campylobacter jejuni bacteria causes severe damage to DNA. Saha C, Mohanraju P, Stubbs A, Dugar G, Hoogstrate Y, Kremers GJ, van Cappellen WA, Horst-Kreft D, Laffeber C, Lebbink JHG, Bruens S, Gaskin D, Beerens D, Klunder M, Joosten R, Demmers JAA, van Gent D, Mouton JW, van der Spek PJ, van der Oost J, van Baarlen P, Louwen R. Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi: 10.1126/sciadv.aaz4849. eCollection 2020 Jun. PubMed

Associated Plasmids

Kinetic Modifications of C4 PEPC Are Qualitatively Convergent, but Larger in Panicum Than in Flaveria. Moody NR, Christin PA, Reid JD. Front Plant Sci. 2020 Jul 3;11:1014. doi: 10.3389/fpls.2020.01014. eCollection 2020. PubMed
A molecular sensor to quantify the localization of proteins, DNA and nanoparticles in cells. FitzGerald LI, Aurelio L, Chen M, Yuen D, Rennick JJ, Graham B, Johnston APR. Nat Commun. 2020 Sep 8;11(1):4482. doi: 10.1038/s41467-020-18082-8. PubMed

Associated Plasmids

PASTA: PARP activity screening and inhibitor testing assay. Kirby IT, Sanderson DJ, Cohen MS. STAR Protoc. 2021 Feb 18;2(1):100344. doi: 10.1016/j.xpro.2021.100344. eCollection 2021 Mar 19. PubMed
Cryo-EM structure of mammalian RNA polymerase II in complex with human RPAP2. Fianu I, Dienemann C, Aibara S, Schilbach S, Cramer P. Commun Biol. 2021 May 21;4(1):606. doi: 10.1038/s42003-021-02088-z. PubMed
Structural characterization of DynU16, a START/Bet v1-like protein involved in dynemicin biosynthesis. Alvarado SK, Miller MD, Bhardwaj M, Thorson JS, Van Lanen SG, Phillips GN Jr. Acta Crystallogr F Struct Biol Commun. 2021 Oct 1;77(Pt 10):328-333. doi: 10.1107/S2053230X21008943. Epub 2021 Sep 21. PubMed
Target site selection and remodelling by type V CRISPR-transposon systems. Querques I, Schmitz M, Oberli S, Chanez C, Jinek M. Nature. 2021 Nov;599(7885):497-502. doi: 10.1038/s41586-021-04030-z. Epub 2021 Nov 10. PubMed
Rational design of selective inhibitors of PARP4. Kirby IT, Person A, Cohen M. RSC Med Chem. 2021 Oct 4;12(11):1950-1957. doi: 10.1039/d1md00195g. eCollection 2021 Nov 17. PubMed
Molecular architecture of the human tRNA ligase complex. Kroupova A, Ackle F, Asanovic I, Weitzer S, Boneberg FM, Faini M, Leitner A, Chui A, Aebersold R, Martinez J, Jinek M. Elife. 2021 Dec 2;10. pii: 71656. doi: 10.7554/eLife.71656. PubMed
The TFAMoplex-Conversion of the Mitochondrial Transcription Factor A into a DNA Transfection Agent. Burger M, Kaelin S, Leroux JC. Adv Sci (Weinh). 2022 Mar;9(8):e2104987. doi: 10.1002/advs.202104987. Epub 2022 Jan 17. PubMed
Multivalent Interactions Drive the Toxoplasma AC9:AC10:ERK7 Complex To Concentrate ERK7 in the Apical Cap. Back PS, O'Shaughnessy WJ, Moon AS, Dewangan PS, Reese ML, Bradley PJ. mBio. 2021 Feb 22;13(1):e0286421. doi: 10.1128/mbio.02864-21. Epub 2022 Feb 8. PubMed
Monitoring Nuclease Activity by X-Ray Scattering Interferometry Using Gold Nanoparticle-Conjugated DNA. Rosenberg DJ, Syed A, Tainer JA, Hura GL. Methods Mol Biol. 2022;2444:183-205. doi: 10.1007/978-1-0716-2063-2_12. PubMed
Structure and activity of a bacterial defense-associated 3'-5' exonuclease. Liang Q, Richey ST, Ur SN, Ye Q, Lau RK, Corbett KD. Protein Sci. 2022 Jul;31(7):e4374. doi: 10.1002/pro.4374. PubMed
Fip1 is a multivalent interaction scaffold for processing factors in human mRNA 3' end biogenesis. Muckenfuss LM, Migenda Herranz AC, Boneberg FM, Clerici M, Jinek M. Elife. 2022 Sep 8;11:e80332. doi: 10.7554/eLife.80332. PubMed
Allosteric activation of CRISPR-Cas12a requires the concerted movement of the bridge helix and helix 1 of the RuvC II domain. Worle E, Newman A, D'Silva J, Burgio G, Grohmann D. Nucleic Acids Res. 2022 Sep 23;50(17):10153-10168. doi: 10.1093/nar/gkac767. PubMed
IMC10 and LMF1 mediate mitochondrial morphology through mitochondrion-pellicle contact sites in Toxoplasma gondii. Oliveira Souza RO, Jacobs KN, Back PS, Bradley PJ, Arrizabalaga G. J Cell Sci. 2022 Nov 15;135(22):jcs260083. doi: 10.1242/jcs.260083. Epub 2022 Nov 15. PubMed
DNA-Stimulated Liquid-Liquid phase separation by eukaryotic topoisomerase ii modulates catalytic function. Jeong J, Lee JH, Carcamo CC, Parker MW, Berger JM. Elife. 2022 Nov 7;11:e81786. doi: 10.7554/eLife.81786. PubMed
The miniature CRISPR-Cas12m effector binds DNA to block transcription. Wu WY, Mohanraju P, Liao C, Adiego-Perez B, Creutzburg SCA, Makarova KS, Keessen K, Lindeboom TA, Khan TS, Prinsen S, Joosten R, Yan WX, Migur A, Laffeber C, Scott DA, Lebbink JHG, Koonin EV, Beisel CL, van der Oost J. Mol Cell. 2022 Dec 1;82(23):4487-4502.e7. doi: 10.1016/j.molcel.2022.11.003. Epub 2022 Nov 24. PubMed
Structural basis for the assembly of the type V CRISPR-associated transposon complex. Schmitz M, Querques I, Oberli S, Chanez C, Jinek M. Cell. 2022 Dec 22;185(26):4999-5010.e17. doi: 10.1016/j.cell.2022.11.009. Epub 2022 Nov 25. PubMed

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