Characterizing a thermostable Cas9 for bacterial genome editing and silencing.
Mougiakos I, Mohanraju P, Bosma EF, Vrouwe V, Finger Bou M, Naduthodi MIS, Gussak A, Brinkman RBL, van Kranenburg R, van der Oost J
Nat Commun. 2017 Nov 21;8(1):1647. doi: 10.1038/s41467-017-01591-4. PubMed
Mougiakos I, Mohanraju P, Bosma EF, Vrouwe V, Finger Bou M, Naduthodi MIS, Gussak A, Brinkman RBL, van Kranenburg R, van der Oost J
Nat Commun. 2017 Nov 21;8(1):1647. doi: 10.1038/s41467-017-01591-4. PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 100981 | pThermoCas9_ctrl | Expresses ThermoCas9 and its sgRNA module |
| 100982 | pThermoCas9i_ctrl | Expresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA module |