pCDH-TfR-mApple
(Plasmid #179384)

• Purpose: Lentiviral vector for expression of Human Transferrin receptor-1 fused mApple
• Depositing Lab: Angus Johnston
• Publication: Rennick et al Nat Commun. 2022 Oct 12;13(1):6023. doi: 10.1038/s41467-022-33348-z.

Backbone

• Vector backbone: pCDH
• Backbone manufacturer: System Biosciences
• Backbone size w/o insert (bp): 7644
• Total vector size (bp): 10695
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Transferrin receptor 1 - mApple
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 3039
• GenBank ID: 7037
• Entrez Gene: TFRC (a.k.a. CD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90)
• Promoter: EF1
• Tag / Fusion Protein:
  • mApple (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: caagctgtgaccggcg
• 3′ sequencing primer: gcttcggccagtaacgtta

Resource Information

• A portion of this plasmid was derived from a plasmid made by: mApple and TfR DNA were obtained from mApple-Lysosomes-20 (RRID:Addgene_54921) and mCherry-TFR-20 (RRID:Addgene_55144) respectively, which were a gift from Michael Davidson.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2022.04.13.488146 for bioRxiv preprint.