166,708 results

1. CDK1as_T2A_Zeo

   Plasmid

   #118596

   Purpose

   One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.

   Depositor

   William Earnshaw

   Article

   Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

   Insert

   CDK1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pH2B-miRFP713-N1

   Plasmid

   #197230

   Purpose

   Expresses the fusion protein of H2B-miRFP713 in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   H2B-miRFP713
   

   Expression

   Mammalian

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHIPH4

   Plasmid

   #117685

   Purpose

   Hansenula polymorpha expression plasmid

   Depositor

   Ida van der Klei

   Insert

   Alcohol Oxidase promoter
   

   Expression

   Yeast

   Promoter

   Alcohol Oxidase

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSBY1_FnCpf1cg

   Plasmid

   #104622

   Purpose

   CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn CPf1

   Depositor

   Sheng Yang

   Article

   Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

   Insert

   FnCpf1
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   codon-optimized C.glutamicum

   Promoter

   hsp60

   Available Since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pICSL90003

   Plasmid

   #68262

   Purpose

   Level 0 Golden Gate Part: Promoter U6 (Triticum aestivum)

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter: U6 (Triticum aestivum)
   

   Use

   Synthetic Biology

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF2857

   Plasmid

   #143642

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ID4 (ID4 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. PINK1 gRNA (BRDN0001145357)

   Plasmid

   #78036

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PINK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PINK1 (PINK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBT13-splitC

   Plasmid

   #122596

   Purpose

   clone M13 phage by SapI Golden Gate

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   M13 bacteriophage genes
   

   Expression

   Bacterial

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWB602-RBCS-tagRFP

   Plasmid

   #213868

   Purpose

   Binary vector for the expression of RBCS fused to the tagRFPn plants (for Agrobacterium-mediated genetic transformation.)

   Depositor

   Yutaka Kodama

   Article

   Ichikawa et al Plant Physiol. 2023 Oct 4:kiad528. doi:

   Insert

   RIBULOSE BISPHOSPHATE CARBOXYLASE SMALL CHAIN 1A (RBCS) (RBCS1A Mustard Weed)
   

   Expression

   Bacterial and Plant

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWB508-cTP-mCherry

    Plasmid

    #213862

    Purpose

    Binary vector for the expression of mCherry flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

    Insert

    Plastid transit peptide fused mCherry-UnaG
    

    Expression

    Bacterial and Plant

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCIS.MTRIA-AVP

    Plasmid

    #184600

    Purpose

    For expression of a fluorescent sensor for vasopressin in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-AVP
    

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. miniSOG-H2B-C-10

    Plasmid

    #57769

    Purpose

    Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    H2B (H2BC11 Human)
    

    Tags

    miniSOG

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pCDNA3 Flag p38 gamma

    Plasmid

    #20353

    Depositor

    Roger Davis

    Article

    Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

    Insert

    pDCNA3 Flag p38 gamma (Mapk12 Rat)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR

    Plasmid

    #162335

    Purpose

    Lentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    S. pyogenes sgRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHG6

    Plasmid

    #188254

    Purpose

    Integrating plasmid contains the BT1 int/attP locus and the erythromycin-resistance gene for selection

    Depositor

    Hong Gao, Maggie Smith

    Article

    Gao et al Access Microbiol. 2021 Dec 8;3(12):00029

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    act1p/actII-orf4

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBT138b-proD

    Plasmid

    #138516

    Purpose

    P3e

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    T7-RNAP
    

    Use

    Synthetic Biology

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-tetON-NOTUM-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204188

    Purpose

    Doxycycline inducible lentiviral vector for human NOTUM overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NOTUM (NOTUM Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TetON

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCIS.MTRIA-OT

    Plasmid

    #184596

    Purpose

    For expression of a fluorescent sensor for oxytocin in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-OT
    

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAT9658-sgRNA-mCherry

    Plasmid

    #162987

    Purpose

    sgRNA cloning plasmid

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    exchangeable cassette
    

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    U6

    Available Since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKAM-ME-Flag-BFP

    Plasmid

    #101868

    Purpose

    Lentiviral Mds1-Evi-1-Flag with dual BFP and puro markers

    Depositor

    Archibald Perkins

    Insert

    Mds1-Evi-1 (Mecom Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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