161,186 results

1. pGEX-4T1_ATG4B_BamHI_NotI

   Plasmid

   #190862

   Purpose

   Plasmid for the expression and purification of of ATG4B. Internal reference: SMC1392

   Depositor

   Sascha Martens

   Article

   Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Insert

   ATG4B (ATG4B Human)
   

   Use

   Tags

   GST, Thrombin Cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1 mCherry

   Plasmid

   #128073

   Purpose

   puro exchanged to mCherry

   Depositor

   Oskar Laur

   Insert

   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   no

   Promoter

   Available since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. N1-Tq-Ca-FLITS

   Plasmid

   #191454

   Purpose

   Genetically encoded calcium sensor (GECI) Tq-Ca-FLITS that reports with lifetime and intensity changes

   Depositor

   Dorus Gadella

   Article

   van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

   Insert

   Tq-Ca-FLITS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF2461

   Plasmid

   #142091

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   IRF7 (IRF7 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUW-M2rtTA

   Plasmid

   #20342

   Purpose

   Lentiviral plasmid expressing the reverse tetracycline transactivator

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

   Insert

   M2rtTA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-CalfluxVTN

   Plasmid

   #83926

   Purpose

   Bioluminescent Reporter of Intracellular Calcium

   Depositor

   Carl Johnson

   Article

   Yang et al Nat Commun. 2016 Oct 27;7:13268. doi: 10

   Insert

   CalfluxVTN
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available since

   Oct. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Plasmid L5 attB::Pleft* mWasabi

   Plasmid

   #169409

   Purpose

   Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mWasabi

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Inserts

   pLeft
   mWasabi
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. LCC-ICCG_pET21b

   Plasmid

   #215408

   Purpose

   Expression construct for LCC-ICCG gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   LCC-ICCG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   F243I/D238C/S283C/Y127G

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mCherry-CD81-10

   Plasmid

   #55012

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   CD81 (CD81 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMK382

    Plasmid

    #214373

    Purpose

    Tet-OsTIR1(F74G) (AAVS1 insertion donor)

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    Tet-OsTIR1(F74G)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet ON 3G promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3.1-Nanog

    Plasmid

    #28221

    Depositor

    Linzhao Cheng

    Article

    Chou et al Cell Res. 2011 Jan 18. ():.

    Insert

    Nanog (NANOG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGBKT7-GW

    Plasmid

    #61703

    Purpose

    Gateway compatible Y2H vector - BD

    Depositor

    Yuhai Cui

    Article

    Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRE-mCherry-p2A-bReaches-TS

    Plasmid

    #158701

    Purpose

    Reporter construct

    Depositor

    Alice Ting

    Article

    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

    Insert

    mCherry-p2A-bReaches-TS
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. scramble shRNA

    Plasmid

    #1864

    Purpose

    3rd gen lentiviral negative control vector containing scrambled shRNA

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    scramble
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEB-Tg

    Plasmid

    #28220

    Depositor

    Linzhao Cheng

    Article

    Chou et al Cell Res. 2011 Jan 18. ():.

    Insert

    SV40 large T
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCE-mp53DD

    Plasmid

    #41856

    Purpose

    Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Δ40-903

    Promoter

    CAG

    Available since

    July 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCFD5_w

    Plasmid

    #112645

    Purpose

    Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.

    Depositor

    Michael Boutros

    Insert

    U6:3-tRNA-sgRNA expression cassette
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-GFP

    Plasmid

    #106281

    Purpose

    A version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.

    Depositor

    Jay Shendure

    Article

    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

    Insert

    CMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYS16

    Plasmid

    #202082

    Purpose

    Parent vector for producing universal minicircle donors for mNeonGreen C-terminal knock-in

    Depositor

    Daniel Dickinson

    Article

    Shi et al Development. 2023 May 15;150(10):dev2013

    Insert

    CT mNG-T2A-PuroR Minicircle Parent
    

    Use

    CRISPR

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    None

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. SNAPtag-PTS1

    Plasmid

    #182883

    Purpose

    Express SNAPtag-PTS1 in mammalian cells. SNAPtag targets to peroxisomes

    Depositor

    Pekka Katajisto

    Article

    Bui et al Nat Commun. 2025 Apr 26;16(1):3932. doi:

    Insert

    SNAPtag-PTS1
    

    Use

    Lentiviral

    Tags

    SNAPtag

    Expression

    Mammalian

    Mutation

    no

    Promoter

    CMV

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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