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pCW57-MCS1-P2A-MCS2 (Blast)
(Plasmid #80921)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 80921 Standard format: Plasmid sent in bacteria as agar stab 1 $75
Cloning Grade DNA 80921-DNA.cg 2 µg of cloning grade DNA in Tris buffer
More Information
1 $95

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCW57.1
  • Backbone manufacturer
    Broad Insitute
  • Backbone size (bp) 7542
  • Modifications to backbone
    pCW57-MCS1-2A-MCS2 was modified by replacing puromycin with blasticidin.
  • Vector type
    Mammalian Expression, Lentiviral ; Doxycycline inducible; P2A Self Cleaving Peptide
  • Promoter TRE
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Cloning Information

Resource Information

Information for Cloning Grade DNA (Catalog # 80921-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $95 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Resource Information

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCW57-MCS1-P2A-MCS2 (Blast) was a gift from Adam Karpf (Addgene plasmid # 80921 ; http://n2t.net/addgene:80921 ; RRID:Addgene_80921)
  • For your References section:

    Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression in Cancer. Barger CJ, Branick C, Chee L, Karpf AR. Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 10.3390/cancers11020251. 10.3390/cancers11020251 PubMed 30795624