Purpose(Empty Backbone) All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||80921||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||80921-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
Backbone manufacturerBroad Insitute
- Backbone size (bp) 7542
Modifications to backbonepCW57-MCS1-2A-MCS2 was modified by replacing puromycin with blasticidin.
Vector typeMammalian Expression, Lentiviral ; Doxycycline inducible; P2A Self Cleaving Peptide
- Promoter TRE
Growth in Bacteria
Growth Strain(s)NEB Stable
- Cloning method Restriction Enzyme
- 5′ sequencing primer pCW57.MCS_Seq_F CGTATGTCGAGGTAGGCGTG (Common Sequencing Primers)
Information for Cloning Grade DNA (Catalog # 80921-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCW57-MCS1-P2A-MCS2 (Blast) was a gift from Adam Karpf (Addgene plasmid # 80921 ; http://n2t.net/addgene:80921 ; RRID:Addgene_80921)
For your References section:Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression in Cancer. Barger CJ, Branick C, Chee L, Karpf AR. Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 10.3390/cancers11020251. 10.3390/cancers11020251 PubMed 30795624