Purpose(Empty Backbone) lentiviral backbone
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85133||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||85133-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
- Backbone size (bp) 8614
- Promoter EF1a
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
- Efficient selection of stably transduced cells due to IRES-driven selectable marker.
- Suitable cloning sites are BamHI/EcoRI, BamHI/NotI.
- Compatible with 2nd and 3rd generation lentiviral packaging systems.
Information for Cloning Grade DNA (Catalog # 85133-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
- Not Available to Industry
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLV-EF1a-IRES-Blast was a gift from Tobias Meyer (Addgene plasmid # 85133 ; http://n2t.net/addgene:85133 ; RRID:Addgene_85133)
For your References section:Engulfed cadherin fingers are polarized junctional structures between collectively migrating endothelial cells. Hayer A, Shao L, Chung M, Joubert LM, Yang HW, Tsai FC, Bisaria A, Betzig E, Meyer T. Nat Cell Biol. 2016 Dec;18(12):1311-1323. doi: 10.1038/ncb3438. Epub 2016 Nov 14. 10.1038/ncb3438 PubMed 27842057