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pEF1a-FB-dCas9-puro
(Plasmid #100547)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 100547 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEF1a-FB-puro
  • Backbone size w/o insert (bp) 7064
  • Total vector size (bp) 11244
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    4305
  • Promoter Human EF1a
  • Tag / Fusion Protein
    • FLAG, Biotin-acceptor-site (Avitag) (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTTCCATTTCAGGTGTCGTG
  • 3′ sequencing primer ctggccacctctgcttgt
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    puromycin
  • Species
    Streptomyces alboniger
  • Insert Size (bp)
    600

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF1a-FB-dCas9-puro was a gift from Jian Xu (Addgene plasmid # 100547 ; http://n2t.net/addgene:100547 ; RRID:Addgene_100547)
  • For your References section:

    In Situ Capture of Chromatin Interactions by Biotinylated dCas9. Liu X, Zhang Y, Chen Y, Li M, Zhou F, Li K, Cao H, Ni M, Liu Y, Gu Z, Dickerson KE, Xie S, Hon GC, Xuan Z, Zhang MQ, Shao Z, Xu J. Cell. 2017 Aug 24;170(5):1028-1043.e19. doi: 10.1016/j.cell.2017.08.003. 10.1016/j.cell.2017.08.003 PubMed 28841410