Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

In Situ Capture of Chromatin Interactions by Biotinylated dCas9.
Liu X, Zhang Y, Chen Y, Li M, Zhou F, Li K, Cao H, Ni M, Liu Y, Gu Z, Dickerson KE, Xie S, Hon GC, Xuan Z, Zhang MQ, Shao Z, Xu J
Cell. 2017 Aug 24;170(5):1028-1043.e19. doi: 10.1016/j.cell.2017.08.003.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
100547pEF1a-FB-dCas9-puroExpresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein
100548pEF1a-BirA-V5-neoExpresses C-terminal V5-tagged BirA biotin ligase
100549pSLQ1651-sgRNA(F+E)-sgGal4sgGal4-expressing vector modified from Addgene plasmid #51024