161,186 results

1. pET28a-mCherry-CNA35

   Plasmid

   #61607

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with mCherry

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   mCherry and CNA35
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1/CALR wt-FLAG

   Plasmid

   #204532

   Purpose

   Mammalian expression of human CALR wt-FLAG

   Depositor

   Norio Komatsu

   Article

   Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

   Insert

   CALR wt-FLAG (CALR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PLKO.1-UPF1-CDS

   Plasmid

   #136037

   Purpose

   UPF1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (AAGACACCTATTACACGAAGG)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   UPF1 (UPF1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-Q74

   Plasmid

   #40262

   Depositor

   David Rubinsztein

   Article

   Narain et al J Med Genet. 1999 Oct;36(10):739-46.

   Insert

   HTT partial exon 1 Q74 (HTT Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   expanded poly-Q

   Promoter

   CMV

   Available since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9_T2A_iRFP670 sgAAVS1

   Plasmid

   #192477

   Purpose

   All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a control

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Use

   Tags

   T2A iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBWB162

   Plasmid

   #140634

   Purpose

   pBAV1k-lacI-Trc-mCherry base, lacI repression vector for ADP1

   Depositor

   Keith Tyo

   Article

   Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9_T2A_iRFP670 sgBFP

   Plasmid

   #192478

   Purpose

   All-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Use

   Tags

   T2A iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. T7-VEE-GFP

   Plasmid

   #58977

   Purpose

   expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Insert

   EGFP
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   26S subgenomic promoter

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

   Plasmid

   #197511

   Purpose

   Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
   

   Use

   AAV and CRISPR

   Tags

   BPNLS and BPNLS-NpuC(intein)

   Expression

   Mutation

   C-terminal mutations of SpRY(L1111R/D1135L/S1136W…

   Promoter

   Cbh

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNK497

    Plasmid

    #219754

    Purpose

    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)

    Plasmid

    #197510

    Purpose

    Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
    

    Use

    AAV and CRISPR

    Tags

    BPNLS and NpuN(intein)-BPNLS

    Expression

    Mutation

    ABE8e mutations in TadA and nSpRY(D10A/A61R)

    Promoter

    Cbh

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Olig001

    Plasmid

    #170716

    Purpose

    Olig001 encodes a novel AAV capsid that exhibits preferential oligodendrocyte tropism in the CNS

    Depositor

    Thomas McCown

    Article

    Powell et al Gene Ther. 2016 Nov;23(11):807-814. doi:

    Insert

    Olig001 capsid gene / rep 2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSCV-IRES-GFP

    Plasmid

    #20672

    Purpose

    Depositor

    Tannishtha Reya

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-EML4-ALK variant 1

    Plasmid

    #183828

    Purpose

    Express EML4-ALK fusion variant 1 (E13;A20) in mammalian cells

    Depositor

    Aaron Hata

    Article

    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

    Inserts

    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of EML4 exon 1-13 and ALK exon…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVS1-Tet-OsTIR1(F74G)-V5

    Plasmid

    #158664

    Purpose

    Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    Tet-OsTIR1(F74G) AAVS1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    F74G

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX_DSB_Spectrum-V2

    Plasmid

    #192475

    Purpose

    Lentiviral DSB Spectrum V2 Reporter System

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    BFP1.2, incompleteGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1GFP/RNase H1 D210N

    Plasmid

    #174448

    Purpose

    Express GFP-tagged RNase H1 D210N in E. coli.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    RNase H1 (RNASEH1 Human)
    

    Use

    Tags

    His-tag, GFP

    Expression

    Bacterial

    Mutation

    D210N

    Promoter

    T7 promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCF159_BRL

    Plasmid

    #225962

    Purpose

    CMV-Intron-BRL (env protein). Expresses BRL (BaEVRLess) env for VLP production.

    Depositor

    Christof Fellmann

    Article

    Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

    Insert

    CMV-Intron-BRL (env protein)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PB-CMV-MCS-Puro-EGFP

    Plasmid

    #219795

    Purpose

    Mammalian expression plasmids using piggyBac Transposon System

    Depositor

    Keiichi Nakayama

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-N2-COL1A1

    Plasmid

    #66602

    Purpose

    Expresses procollagen 1 alpha 1 as a GFP fusion

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

    Insert

    COL1A1 (COL1A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge