166,778 results

1. pQmod2S-GG

   Plasmid

   #191346

   Purpose

   Clostridium expression vector (pBP1 origin, specR) with drop-out Plac-RFP cassette flanked with BsaI sites

   Depositor

   Andrew Tolonen

   Article

   Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

   Insert

   Plac-RFP (IPTG-inducible red fluorescent protein)
   

   Expression

   Bacterial

   Promoter

   Plac

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. MAC_N_TOM22

   Plasmid

   #111697

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   TOM22 (TOMM22 Human)
   

   Expression

   Mammalian

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-eArch3.0-mRuby2-ST

   Plasmid

   #109135

   Purpose

   Archaerhodopsin fused to mRuby2 and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   eArch3.0-mRuby2-ST
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLPC-L647RLaminA

   Plasmid

   #69060

   Purpose

   encodes a lamin A mutant with Leucine 647 mutated to an Arginin

   Depositor

   Gerardo Ferbeyre

   Article

   Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

   Insert

   LaminA (LMNA Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Lamin A with Leucine 647 mutated to Arginine

   Promoter

   CMV

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pTF101.1gw1

   Plasmid

   #134768

   Purpose

   A binary plasmid for gateway 1-Entry in pTF101.1 background (attR1 & attR2) containing double CaMV 35S promoter and TEV enhancer to drive bialaphos resistance gene (bar) for plant transformation

   Depositor

   Kan Wang

   Article

   et al Euphytica (2004) 136: 167.

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmVen-RecQL4

   Plasmid

   #211401

   Purpose

   mammalian expression of human RecQL4 N-terminally fused to mVenus

   Depositor

   Helmut Pospiech

   Insert

   RecQL4 (RECQL4 Human)
   

   Tags

   mVenus

   Expression

   Mammalian

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIB3

   Plasmid

   #25452

   Depositor

   Benjamin Glick

   Article

   Sears et al Yeast. 1998 Jun 15;14(8):783-90.

   Insert

   Pichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)
   

   Use

   Pichia pastoris expression

   Available Since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. HP-Cas9hGem-Ade2-LEU2-LINEAR

   Plasmid

   #174839

   Purpose

   pRS414 backbone containing LINEAR fragment targeting ADE2 in H. polymorpha

   Depositor

   Zengyi Shao

   Article

   Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

   Insert

   HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial and Yeast

   Promoter

   TDH3p

   Available Since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBXNH3

   Plasmid

   #47067

   Purpose

   FX cloning E.coli expression vector with araBAD promoter and N-terminal 10x His tag and 3C protease cleavage site

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Tags

   3C protease site (PreScission site) and decaHis (…

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF0732

    Plasmid

    #141675

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PROX2 (PROX2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. mEos2-VASP-5

    Plasmid

    #57433

    Purpose

    Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    VASP
    

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    A209T in VASP

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pHIS-MDH1-HIS

    Plasmid

    #184558

    Purpose

    Bacterial expression of human MDH1 with HIS Tag

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Insert

    Malate dehydrogenase 1 (MDH1 Human)
    

    Tags

    6xHIS

    Expression

    Bacterial

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF1510

    Plasmid

    #144365

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    STAT5A (STAT5A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. GST-RB (829-928)

    Plasmid

    #31498

    Depositor

    Julien Sage

    Article

    Saddic et al J Biol Chem. 2010 Nov 26. 285(48):37733-

    Insert

    RB (RB1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains amino acids 829-928

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

15. GST-RB (736-840)

    Plasmid

    #31497

    Depositor

    Julien Sage

    Article

    Saddic et al J Biol Chem. 2010 Nov 26. 285(48):37733-

    Insert

    RB (RB1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains amino acids 736-840

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

16. ORAI1-YFP T184M

    Plasmid

    #114181

    Purpose

    ORAI1 channel with C-terminal YFP, carrying the mutation T184M in the protein 3rd transmembrane domain, responsible for the channel overactivity and associated with tubular aggregate myopathy (TAM).

    Depositor

    Nicolas Demaurex

    Article

    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

    Insert

    ORAI1-YFP T184M (ORAI1 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    T184M

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. GST-RB (561-673)

    Plasmid

    #31495

    Depositor

    Julien Sage

    Article

    Saddic et al J Biol Chem. 2010 Nov 26. 285(48):37733-

    Insert

    RB (RB1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains amino acids 561-673

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

18. tubulin-YeNL_pcDNA3

    Plasmid

    #89519

    Purpose

    Yellow color variant of bright luminescent protein, fused to b-tubulin

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    YeNL
    

    Tags

    b-tubulin

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. GST-RB (654-752)

    Plasmid

    #31496

    Depositor

    Julien Sage

    Article

    Saddic et al J Biol Chem. 2010 Nov 26. 285(48):37733-

    Insert

    RB (RB1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains amino acids 654-752

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

20. STARR-seq luciferase validation vector_ORI_IGF1R

    Plasmid

    #99306

    Purpose

    Luciferase validation vector with IGF1R enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Insert

    enhancer; chr15: 99439352 -99440791
    

    Use

    Luciferase; Starr-seq luciferase validation vector

    Expression

    Mammalian

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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