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(Plasmid #122205)


Item Catalog # Description Quantity Price (USD)
Plasmid 122205 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Modifications to backbone
    Added MeCP2 fusion to dCas9-KRAB and swapped the selection marker from BFP to BSD.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number


  • Gene/Insert name
  • Alt name
  • Species
  • Insert Size (bp)
  • Mutation
    Cas9 (D10A, H840A)
  • Tag / Fusion Protein
    • KRAB-MeCP2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site SbfI (unknown if destroyed)
  • 5′ sequencing primer AACGGCAGCAGCGGATC
  • 3′ sequencing primer CGACCTGCAGGCATGCAA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lenti_dCas9-KRAB-MeCP2 was a gift from Andrea Califano (Addgene plasmid # 122205 ; ; RRID:Addgene_122205)
  • For your References section:

    Interrogation of genome-wide, experimentally dissected gene regulatory networks reveals mechanisms underlying dynamic cellular state control. Tan X, Worley J, Turunen M, Wong K, Fernández EC, Paull E, Jones S, Wang J, Noh H, Salvatori B, Chavez A, Califano A. bioRxiv 2021.06.28.449297 10.1101/2021.06.28.449297