165,688 results

1. SNX9-pmCherryC1

   Plasmid

   #27678

   Depositor

   Christien Merrifield

   Article

   Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

   Insert

   SNX9 (Snx9 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   May 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs-hSOX2

   Plasmid

   #17218

   Purpose

   Retroviral expression of human SOX2

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

   Insert

   SOX2 (SOX2 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST-FLAG

   Plasmid

   #183511

   Purpose

   Gateway converted, destination vector with N-terminal Flag tag. For mammalian expression.

   Depositor

   Emanuele Panza

   Article

   Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

   Type

   Empty backbone

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-mBATF

   Plasmid

   #34575

   Depositor

   Peter Murray

   Article

   Smith et al J Biol Chem. 2011 Jul 1;286(26):23582-90

   Insert

   BATF (Batf Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pCMV-SpCas9(H840A)-T2A-EGFP

   Plasmid

   #221231

   Purpose

   Express nCas9 nickase (H840A) with GFP reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   SpCas9(H840A)-T2A-EGFP
   

   Expression

   Mammalian

   Mutation

   H840A

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. eGFP-hCOL1A1

   Plasmid

   #140110

   Purpose

   Expresses fluorescently-tagged human Type I procollagen α1 chain with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Insert

   Type I procollagen α1 (COL1A1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   COL1A1 exons 2-3 replaced by fluorescent tag, K20…

   Promoter

   CMV

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF2629

   Plasmid

   #142966

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   INSM2 (INSM2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP NFAT5 (no EGFP)

   Plasmid

   #13627

   Depositor

   Anjana Rao

   Article

   Lopez-Rodriguez et al Proc Natl Acad Sci U S A. 1999 Jun 22. 9

   Insert

   NFAT5 (NFAT5 Human)
   

   Tags

   6xMyc

   Expression

   Mammalian

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2631

   Plasmid

   #144140

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   INSM1 (INSM1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYSD061

    Plasmid

    #212915

    Purpose

    Encodes the S. cerevisiae SUC2 (Invertase) protein (minus signal peptide) as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    SUC2 (SUC2 Budding Yeast)
    

    Expression

    Bacterial

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMOS016: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

    Plasmid

    #163058

    Purpose

    roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    roGFP2-Orp1
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-PGK-KRAS4B(G12V)

    Plasmid

    #35633

    Depositor

    Daniel Haber

    Article

    Singh et al Cell. 2012 Feb 17;148(4):639-50.

    Insert

    K-Ras4B(G12V) (KRAS Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Glycine 12 to Valine

    Promoter

    PGK

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGH335_MS2-AID*Δ-Hygro

    Plasmid

    #85406

    Purpose

    lentiviral expression vector for MS2-AID*Δ fusion protein with hygromycin B selectable marker

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    MS2-AID*Δ and Hygromycin resistance
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pegRNA2_tevopreQ1

    Plasmid

    #212039

    Purpose

    Bacterial expression of the pegRNA for genome editing in E. coli

    Depositor

    Quanjiang Ji

    Article

    Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

    Insert

    pegRNA
    

    Use

    CRISPR

    Promoter

    J23119

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Sp_PE

    Plasmid

    #212038

    Purpose

    Bacterial expression of the PE2 proteins for genome editing in E. coli

    Depositor

    Quanjiang Ji

    Article

    Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

    Insert

    PE2
    

    Use

    CRISPR

    Promoter

    L-arabinose-inducible promoter PBAD

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-puro /BRAF-V600E

    Plasmid

    #131724

    Purpose

    Mammalian Expression of Flag tagged BRAF

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    V600E

    Promoter

    CMV

    Available Since

    Feb. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pdCas9-humanized

    Plasmid

    #44246

    Purpose

    Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    dead Cas9 with 3X NLS
    

    Use

    CRISPR and Retroviral; Vector

    Tags

    3x NLS

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    MSCV LTR promoter

    Available Since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXs-hKLF4

    Plasmid

    #17219

    Purpose

    Retroviral expression of human KLF4

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-BRCA1

    Plasmid

    #119007

    Purpose

    Mammalian expression of human BRCA1 and BFP

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    BRCA1 (BRCA1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. mNeon-Vamp2

    Plasmid

    #186619

    Purpose

    Encodes Vamp 2 fused to mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

    Insert

    Vesicle Associated Membrane Protein 2 (VAMP2 Human)
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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