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mTORC1 senses lysosomal amino acids through an inside-out mechanism that requires the vacuolar H-ATPase.
Zoncu R, Bar-Peled L, Efeyan A, Wang S, Sancak Y, Sabatini DM
Science. 2011 Nov 4;334(6056):678-83.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
34611LAMP1-mRFP-FLAG3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tag
87931pRK5-FLAG-ATP6V0D1FLAG-tagged overexpression
87932pRK5-ATP6V1D-HAGSTC-term HA-GST tagged overexpression
88856pRK5-myc-PAT1myc-TAG overexpression

Antibodies from Article