pGL3-U6-sgRNA-PGK-puromycin Citations (82)
Originally described in: Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects.Shen B, Zhang W, Zhang J, Zhou J, Wang J, Chen L, Wang L, Hodgkins A, Iyer V, Huang X, Skarnes WC Nat Methods. 2014 Mar 2. doi: 10.1038/nmeth.2857. PubMed Journal
Articles Citing pGL3-U6-sgRNA-PGK-puromycin
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| Pseudovirus-based neuraminidase inhibition assays reveal potential H5N1 drug-resistant mutations. Lu Y, Jiang T. Protein Cell. 2013 May;4(5):356-63. doi: 10.1007/s13238-013-2125-y. Epub 2013 Apr 15. PubMed |
| Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos. Niu Y, Shen B, Cui Y, Chen Y, Wang J, Wang L, Kang Y, Zhao X, Si W, Li W, Xiang AP, Zhou J, Guo X, Bi Y, Si C, Hu B, Dong G, Wang H, Zhou Z, Li T, Tan T, Pu X, Wang F, Ji S, Zhou Q, Huang X, Ji W, Sha J. Cell. 2014 Feb 13;156(4):836-43. doi: 10.1016/j.cell.2014.01.027. Epub 2014 Jan 30. PubMed |
| sgRNAcas9: a software package for designing CRISPR sgRNA and evaluating potential off-target cleavage sites. Xie S, Shen B, Zhang C, Huang X, Zhang Y. PLoS One. 2014 Jun 23;9(6):e100448. doi: 10.1371/journal.pone.0100448. eCollection 2014. PubMed |
| An efficient genotyping method for genome-modified animals and human cells generated with CRISPR/Cas9 system. Zhu X, Xu Y, Yu S, Lu L, Ding M, Cheng J, Song G, Gao X, Yao L, Fan D, Meng S, Zhang X, Hu S, Tian Y. Sci Rep. 2014 Sep 19;4:6420. doi: 10.1038/srep06420. PubMed |
| Dkk3 prevents familial dilated cardiomyopathy development through Wnt pathway. Lu D, Bao D, Dong W, Liu N, Zhang X, Gao S, Ge W, Gao X, Zhang L. Lab Invest. 2016 Feb;96(2):239-48. doi: 10.1038/labinvest.2015.145. Epub 2015 Dec 7. PubMed |
| GPI Anchor Modification Machinery Deficiency is Responsible for the Formation of Pro-PrP in BxPC-3 and Increases Cancer Cell Motility. Yang L, Gao Z, Hu L, Wu G, Yang X, Zhang L, Zhu Y, Wong BS, Xin W, Sy MS, Li C. J Biol Chem. 2015 Dec 18. pii: jbc.M115.705830. PubMed |
| CRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells from cancer patients. Su S, Hu B, Shao J, Shen B, Du J, Du Y, Zhou J, Yu L, Zhang L, Chen F, Sha H, Cheng L, Meng F, Zou Z, Huang X, Liu B. Sci Rep. 2016 Jan 28;6:20070. doi: 10.1038/srep20070. PubMed |
Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome.
Wood LW, Cox NI, Phelps CA, Lai SC, Poddar A, Talbot C Jr, Mu D.
Sci Rep. 2016 Feb 25;6:19857. doi: 10.1038/srep19857.
PubMed
Associated Plasmids |
| CRISPR-Cas9 mediated genetic engineering for the purification of the endogenous integrator complex from mammalian cells. Baillat D, Russell WK, Wagner EJ. Protein Expr Purif. 2016 Dec;128:101-8. doi: 10.1016/j.pep.2016.08.011. Epub 2016 Aug 19. PubMed |
| MicroRNA-202 maintains spermatogonial stem cells by inhibiting cell cycle regulators and RNA binding proteins. Chen J, Cai T, Zheng C, Lin X, Wang G, Liao S, Wang X, Gan H, Zhang D, Hu X, Wang S, Li Z, Feng Y, Yang F, Han C. Nucleic Acids Res. 2016 Dec 19. pii: gkw1287. doi: 10.1093/nar/gkw1287. PubMed |
| CRISPR-Cas9-mediated disruption of PD-1 on human T cells for adoptive cellular therapies of EBV positive gastric cancer. Su S, Zou Z, Chen F, Ding N, Du J, Shao J, Li L, Fu Y, Hu B, Yang Y, Sha H, Meng F, Wei J, Huang X, Liu B. Oncoimmunology. 2016 Nov 22;6(1):e1249558. doi: 10.1080/2162402X.2016.1249558. eCollection 2017. PubMed |
| Isolated polycystic liver disease genes define effectors of polycystin-1 function. Besse W, Dong K, Choi J, Punia S, Fedeles SV, Choi M, Gallagher AR, Huang EB, Gulati A, Knight J, Mane S, Tahvanainen E, Tahvanainen P, Sanna-Cherchi S, Lifton RP, Watnick T, Pei YP, Torres VE, Somlo S. J Clin Invest. 2017 May 1;127(5):1772-1785. doi: 10.1172/JCI90129. Epub 2017 Apr 4. PubMed |
| CRISPR/Cas9-mediated ApoE-/- and LDLR-/- double gene knockout in pigs elevates serum LDL-C and TC levels. Huang L, Hua Z, Xiao H, Cheng Y, Xu K, Gao Q, Xia Y, Liu Y, Zhang X, Zheng X, Mu Y, Li K. Oncotarget. 2017 Jun 6;8(23):37751-37760. doi: 10.18632/oncotarget.17154. PubMed |
| RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice. Jiang L, Li T, Zhang X, Zhang B, Yu C, Li Y, Fan S, Jiang X, Khan T, Hao Q, Xu P, Nadano D, Huleihel M, Lunenfeld E, Wang PJ, Zhang Y, Shi Q. Curr Biol. 2017 May 22;27(10):1498-1505.e6. doi: 10.1016/j.cub.2017.04.017. Epub 2017 May 11. PubMed |
| Nuclear carbonic anhydrase 6B associates with PRMT5 to epigenetically promote IL-12 expression in innate response. Xu J, Xu X, Wang B, Ma Y, Zhang L, Xu H, Hu Y, Wu J, Cao X. Proc Natl Acad Sci U S A. 2017 Aug 8;114(32):8620-8625. doi: 10.1073/pnas.1700917114. Epub 2017 Jul 24. PubMed |
| Prion protein is required for tumor necrosis factor alpha (TNFalpha)-triggered nuclear factor kappaB (NF-kappaB) signaling and cytokine production. Wu GR, Mu TC, Gao ZX, Wang J, Sy MS, Li CY. J Biol Chem. 2017 Nov 17;292(46):18747-18759. doi: 10.1074/jbc.M117.787283. Epub 2017 Sep 12. PubMed |
| DNA Ligase IV Guides End-Processing Choice during Nonhomologous End Joining. Conlin MP, Reid DA, Small GW, Chang HH, Watanabe G, Lieber MR, Ramsden DA, Rothenberg E. Cell Rep. 2017 Sep 19;20(12):2810-2819. doi: 10.1016/j.celrep.2017.08.091. PubMed |
| Engineered cells for costimulatory enhancement combined with IL-21 enhance the generation of PD-1-disrupted CTLs for adoptive immunotherapy. Shao J, Xu Q, Su S, Meng F, Zou Z, Chen F, Du J, Qian X, Liu B. Cell Immunol. 2017 Oct;320:38-45. doi: 10.1016/j.cellimm.2017.09.003. Epub 2017 Sep 7. PubMed |
Multiplex Enhancer Interference Reveals Collaborative Control of Gene Regulation by Estrogen Receptor alpha-Bound Enhancers.
Carleton JB, Berrett KC, Gertz J.
Cell Syst. 2017 Oct 25;5(4):333-344.e5. doi: 10.1016/j.cels.2017.08.011. Epub 2017 Sep 27.
PubMed
Associated Plasmids |
| Relationship between DNA Methylation in the 5' CpG Island of the SLC47A1 (Multidrug and Toxin Extrusion Protein MATE1) Gene and Interindividual Variability in MATE1 Expression in the Human Liver. Tanaka T, Hirota T, Ieiri I. Mol Pharmacol. 2018 Jan;93(1):1-7. doi: 10.1124/mol.117.109553. Epub 2017 Oct 25. PubMed |
| CRISPR-offinder: a CRISPR guide RNA design and off-target searching tool for user-defined protospacer adjacent motif. Zhao C, Zheng X, Qu W, Li G, Li X, Miao YL, Han X, Liu X, Li Z, Ma Y, Shao Q, Li H, Sun F, Xie S, Zhao S. Int J Biol Sci. 2017 Nov 1;13(12):1470-1478. doi: 10.7150/ijbs.21312. eCollection 2017. PubMed |
| Interindividual Differences in the Expression of ATP-Binding Cassette and Solute Carrier Family Transporters in Human Skin: DNA Methylation Regulates Transcriptional Activity of the Human ABCC3 Gene. Takechi T, Hirota T, Sakai T, Maeda N, Kobayashi D, Ieiri I. Drug Metab Dispos. 2018 May;46(5):628-635. doi: 10.1124/dmd.117.079061. Epub 2018 Feb 2. PubMed |
| Generation of GHR-modified pigs as Laron syndrome models via a dual-sgRNAs/Cas9 system and somatic cell nuclear transfer. Yu H, Long W, Zhang X, Xu K, Guo J, Zhao H, Li H, Qing Y, Pan W, Jia B, Zhao HY, Huang X, Wei HJ. J Transl Med. 2018 Feb 27;16(1):41. doi: 10.1186/s12967-018-1409-7. PubMed |
Base editing with a Cpf1-cytidine deaminase fusion.
Li X, Wang Y, Liu Y, Yang B, Wang X, Wei J, Lu Z, Zhang Y, Wu J, Huang X, Yang L, Chen J.
Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102.
PubMed
Associated Plasmids |
| Tumor-Induced Generation of Splenic Erythroblast-like Ter-Cells Promotes Tumor Progression. Han Y, Liu Q, Hou J, Gu Y, Zhang Y, Chen Z, Fan J, Zhou W, Qiu S, Zhang Y, Dong T, Li N, Jiang Z, Zhu H, Zhang Q, Ma Y, Zhang L, Wang Q, Yu Y, Li N, Cao X. Cell. 2018 Apr 19;173(3):634-648.e12. doi: 10.1016/j.cell.2018.02.061. Epub 2018 Mar 29. PubMed |
| Tumor-secreted Pros1 inhibits macrophage M1 polarization to reduce antitumor immune response. Ubil E, Caskey L, Holtzhausen A, Hunter D, Story C, Earp HS. J Clin Invest. 2018 Jun 1;128(6):2356-2369. doi: 10.1172/JCI97354. Epub 2018 Apr 30. PubMed |
BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity.
Jiang W, Feng S, Huang S, Yu W, Li G, Yang G, Liu Y, Zhang Y, Zhang L, Hou Y, Chen J, Chen J, Huang X.
Cell Res. 2018 Jun 6. pii: 10.1038/s41422-018-0052-4. doi: 10.1038/s41422-018-0052-4.
PubMed
Associated Plasmids |
Efficient generation of mouse models of human diseases via ABE- and BE-mediated base editing.
Liu Z, Lu Z, Yang G, Huang S, Li G, Feng S, Liu Y, Li J, Yu W, Zhang Y, Chen J, Sun Q, Huang X.
Nat Commun. 2018 Jun 14;9(1):2338. doi: 10.1038/s41467-018-04768-7.
PubMed
Associated Plasmids |
| Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines. Carleton JB, Berrett KC, Gertz J. J Vis Exp. 2018 Jun 2;(136). doi: 10.3791/57883. PubMed |
| Lipase maturation factor 1 affects redox homeostasis in the endoplasmic reticulum. Roberts BS, Babilonia-Rosa MA, Broadwell LJ, Wu MJ, Neher SB. EMBO J. 2018 Oct 1;37(19). pii: embj.201797379. doi: 10.15252/embj.201797379. Epub 2018 Aug 1. PubMed |
| Correction of the Marfan Syndrome Pathogenic FBN1 Mutation by Base Editing in Human Cells and Heterozygous Embryos. Zeng Y, Li J, Li G, Huang S, Yu W, Zhang Y, Chen D, Chen J, Liu J, Huang X. Mol Ther. 2018 Nov 7;26(11):2631-2637. doi: 10.1016/j.ymthe.2018.08.007. Epub 2018 Aug 14. PubMed |
| BRCA1 Mutation-Specific Responses to 53BP1 Loss-Induced Homologous Recombination and PARP Inhibitor Resistance. Nacson J, Krais JJ, Bernhardy AJ, Clausen E, Feng W, Wang Y, Nicolas E, Cai KQ, Tricarico R, Hua X, DiMarcantonio D, Martinez E, Zong D, Handorf EA, Bellacosa A, Testa JR, Nussenzweig A, Gupta GP, Sykes SM, Johnson N. Cell Rep. 2018 Sep 25;24(13):3513-3527.e7. doi: 10.1016/j.celrep.2018.08.086. PubMed |
| Phosphorylation-Mediated IFN-gammaR2 Membrane Translocation Is Required to Activate Macrophage Innate Response. Xu X, Xu J, Wu J, Hu Y, Han Y, Gu Y, Zhao K, Zhang Q, Liu X, Liu J, Liu B, Cao X. Cell. 2018 Nov 15;175(5):1336-1351.e17. doi: 10.1016/j.cell.2018.09.011. Epub 2018 Oct 11. PubMed |
| Base-Editing-Mediated R17H Substitution in Histone H3 Reveals Methylation-Dependent Regulation of Yap Signaling and Early Mouse Embryo Development. Yang G, Zhou C, Wang R, Huang S, Wei Y, Yang X, Liu Y, Li J, Lu Z, Ying W, Li X, Jing N, Huang X, Yang H, Qiao Y. Cell Rep. 2019 Jan 8;26(2):302-312.e4. doi: 10.1016/j.celrep.2018.12.046. PubMed |
The molecular logic of Nanog-induced self-renewal in mouse embryonic stem cells.
Heurtier V, Owens N, Gonzalez I, Mueller F, Proux C, Mornico D, Clerc P, Dubois A, Navarro P.
Nat Commun. 2019 Mar 7;10(1):1109. doi: 10.1038/s41467-019-09041-z.
PubMed
Associated Plasmids |
| Cancer testis antigen 55 deficiency attenuates colitis-associated colorectal cancer by inhibiting NF-kappaB signaling. Zhao H, Pan WM, Zhang HH, Song Y, Chen J, Xiang Y, Gu B, Li SZ, Du RL, Zhang XD. Cell Death Dis. 2019 Apr 3;10(4):304. doi: 10.1038/s41419-019-1537-x. PubMed |
| MED12 exerts an emerging role in actin-mediated cytokinesis via LIMK2/cofilin pathway in NSCLC. Xu M, Wang F, Li G, Wang X, Fang X, Jin H, Chen Z, Zhang J, Fu L. Mol Cancer. 2019 May 9;18(1):93. doi: 10.1186/s12943-019-1020-4. PubMed |
| Modeling Steatohepatitis in Humans with Pluripotent Stem Cell-Derived Organoids. Ouchi R, Togo S, Kimura M, Shinozawa T, Koido M, Koike H, Thompson W, Karns RA, Mayhew CN, McGrath PS, McCauley HA, Zhang RR, Lewis K, Hakozaki S, Ferguson A, Saiki N, Yoneyama Y, Takeuchi I, Mabuchi Y, Akazawa C, Yoshikawa HY, Wells JM, Takebe T. Cell Metab. 2019 May 30. pii: S1550-4131(19)30247-5. doi: 10.1016/j.cmet.2019.05.007. PubMed |
| Efficient Generation of Pathogenic A-to-G Mutations in Human Tripronuclear Embryos via ABE-Mediated Base Editing. Li G, Liu X, Huang S, Zeng Y, Yang G, Lu Z, Zhang Y, Ma X, Wang L, Huang X, Liu J. Mol Ther Nucleic Acids. 2019 Jun 5;17:289-296. doi: 10.1016/j.omtn.2019.05.021. PubMed |
| Improving Editing Efficiency for the Sequences with NGH PAM Using xCas9-Derived Base Editors. Liu X, Li G, Zhou X, Qiao Y, Wang R, Tang S, Liu J, Wang L, Huang X. Mol Ther Nucleic Acids. 2019 Sep 6;17:626-635. doi: 10.1016/j.omtn.2019.06.024. Epub 2019 Jul 12. PubMed |
| Genetic determinants of cellular addiction to DNA polymerase theta. Feng W, Simpson DA, Carvajal-Garcia J, Price BA, Kumar RJ, Mose LE, Wood RD, Rashid N, Purvis JE, Parker JS, Ramsden DA, Gupta GP. Nat Commun. 2019 Sep 19;10(1):4286. doi: 10.1038/s41467-019-12234-1. PubMed |
| Modelling human hepato-biliary-pancreatic organogenesis from the foregut-midgut boundary. Koike H, Iwasawa K, Ouchi R, Maezawa M, Giesbrecht K, Saiki N, Ferguson A, Kimura M, Thompson WL, Wells JM, Zorn AM, Takebe T. Nature. 2019 Oct;574(7776):112-116. doi: 10.1038/s41586-019-1598-0. Epub 2019 Sep 25. PubMed |
| Evaluation of the effects of sequence length and microsatellite instability on single-guide RNA activity and specificity. Zhao C, Wang Y, Nie X, Han X, Liu H, Li G, Yang G, Ruan J, Ma Y, Li X, Cheng H, Zhao S, Fang Y, Xie S. Int J Biol Sci. 2019 Oct 3;15(12):2641-2653. doi: 10.7150/ijbs.37152. eCollection 2019. PubMed |
| Optimization Strategy for Generating Gene-edited Tibet Minipigs by Synchronized Oestrus and Cytoplasmic Microinjection. Chen B, Gu P, Jia J, Liu W, Liu Y, Liu W, Xu T, Lin X, Lin T, Liu Y, Chen H, Xu M, Yuan J, Zhang J, Zhang Y, Xiao D, Gu W. Int J Biol Sci. 2019 Oct 15;15(12):2719-2732. doi: 10.7150/ijbs.35930. eCollection 2019. PubMed |
| Increasing the targeting scope and efficiency of base editing with Proxy-BE strategy. Liu Y, Li G, Yang G, Gu H, Huang S, Yu W, Qin G, Liu X, Zhou F, Huang X, Wei Y. FEBS Lett. 2019 Dec 14. doi: 10.1002/1873-3468.13719. PubMed |
| APOBEC3A is a prominent cytidine deaminase in breast cancer. Cortez LM, Brown AL, Dennis MA, Collins CD, Brown AJ, Mitchell D, Mertz TM, Roberts SA. PLoS Genet. 2019 Dec 16;15(12):e1008545. doi: 10.1371/journal.pgen.1008545. eCollection 2019 Dec. PubMed |
| Efficient Gene Silencing by Adenine Base Editor-Mediated Start Codon Mutation. Wang X, Liu Z, Li G, Dang L, Huang S, He L, Ma Y, Li C, Liu M, Yang G, Huang X, Zhou F, Ma X. Mol Ther. 2020 Feb 5;28(2):431-440. doi: 10.1016/j.ymthe.2019.11.022. Epub 2019 Nov 29. PubMed |
| A cytokine screen using CRISPR-Cas9 knock-in reporter pig iPS cells reveals that Activin A regulates NANOG. Xu J, Zheng Z, Du X, Shi B, Wang J, Gao D, Zhu Q, Chen X, Han J. Stem Cell Res Ther. 2020 Feb 18;11(1):67. doi: 10.1186/s13287-020-1588-z. PubMed |
| Sepiapterin reductase promotes hepatocellular carcinoma progression via FoxO3a/Bim signaling in a nonenzymatic manner. Wu Y, Du H, Zhan M, Wang H, Chen P, Du D, Liu X, Huang X, Ma P, Peng D, Sun L, Yuan S, Ding J, Lu L, Jiang J. Cell Death Dis. 2020 Apr 20;11(4):248. doi: 10.1038/s41419-020-2471-7. PubMed |
| A Cas-embedding strategy for minimizing off-target effects of DNA base editors. Liu Y, Zhou C, Huang S, Dang L, Wei Y, He J, Zhou Y, Mao S, Tao W, Zhang Y, Yang H, Huang X, Chi T. Nat Commun. 2020 Nov 27;11(1):6073. doi: 10.1038/s41467-020-19690-0. PubMed |
| Cytosine Base Editor (hA3A-BE3-NG)-Mediated Multiple Gene Editing for Pyramid Breeding in Pigs. Wang Y, Bi D, Qin G, Song R, Yao J, Cao C, Zheng Q, Hou N, Wang Y, Zhao J. Front Genet. 2020 Nov 16;11:592623. doi: 10.3389/fgene.2020.592623. eCollection 2020. PubMed |
| Generation of PRMT6 homozygous knockout human embryonic stem cell lines. Li T, Jin W, He Y, Zhou Z. Stem Cell Res. 2020 Dec 28;50:102136. doi: 10.1016/j.scr.2020.102136. PubMed |
| Abnormal neocortex arealization and Sotos-like syndrome-associated behavior in Setd2 mutant mice. Xu L, Zheng Y, Li X, Wang A, Huo D, Li Q, Wang S, Luo Z, Liu Y, Xu F, Wu X, Wu M, Zhou Y. Sci Adv. 2021 Jan 1;7(1). pii: 7/1/eaba1180. doi: 10.1126/sciadv.aba1180. Print 2021 Jan. PubMed |
| CRISPR/Cas9-mediated correction of MITF homozygous point mutation in a Waardenburg syndrome 2A pig model. Yao J, Wang Y, Cao C, Song R, Bi D, Zhang H, Li Y, Qin G, Hou N, Zhang N, Zhang J, Guo W, Yang S, Wang Y, Zhao J. Mol Ther Nucleic Acids. 2021 Apr 16;24:986-999. doi: 10.1016/j.omtn.2021.04.009. eCollection 2021 Jun 4. PubMed |
| Base editing-mediated perturbation of endogenous PKM1/2 splicing facilitates isoform-specific functional analysis in vitro and in vivo. Lin J, Wu S, Shen Q, Liu J, Huang S, Peng G, Qiao Y. Cell Prolif. 2021 Aug;54(8):e13096. doi: 10.1111/cpr.13096. Epub 2021 Jul 9. PubMed |
| Editing Properties of Base Editors with SpCas9-NG in Discarded Human Tripronuclear Zygotes. Liu X, Zhou X, Li G, Huang S, Sun W, Sun Q, Li L, Huang X, Liu J, Wang L. CRISPR J. 2021 Oct;4(5):710-727. doi: 10.1089/crispr.2021.0036. PubMed |
| IKZF3 deficiency potentiates chimeric antigen receptor T cells targeting solid tumors. Zou Y, Liu B, Li L, Yin Q, Tang J, Jing Z, Huang X, Zhu X, Chi T. Cancer Lett. 2022 Jan 1;524:121-130. doi: 10.1016/j.canlet.2021.10.016. Epub 2021 Oct 20. PubMed |
| TM6SF2/PNPLA3/MBOAT7 Loss-of-Function Genetic Variants Impact on NAFLD Development and Progression Both in Patients and in In Vitro Models. Longo M, Meroni M, Paolini E, Erconi V, Carli F, Fortunato F, Ronchi D, Piciotti R, Sabatini S, Macchi C, Alisi A, Miele L, Soardo G, Comi GP, Valenti L, Ruscica M, Fracanzani AL, Gastaldelli A, Dongiovanni P. Cell Mol Gastroenterol Hepatol. 2022;13(3):759-788. doi: 10.1016/j.jcmgh.2021.11.007. Epub 2021 Nov 23. PubMed |
| Generation and characterization of stable pig pregastrulation epiblast stem cell lines. Zhi M, Zhang J, Tang Q, Yu D, Gao S, Gao D, Liu P, Guo J, Hai T, Gao J, Cao S, Zhao Z, Li C, Weng X, He M, Chen T, Wang Y, Long K, Jiao D, Li G, Zhang J, Liu Y, Lin Y, Pang D, Zhu Q, Chen N, Huang J, Chen X, Yao Y, Yang J, Xie Z, Huang X, Liu M, Zhang R, Li Q, Miao Y, Tian J, Huang X, Ouyang H, Liu B, Xie W, Zhou Q, Wei H, Liu Z, Zheng C, Li M, Han J. Cell Res. 2022 Apr;32(4):383-400. doi: 10.1038/s41422-021-00592-9. Epub 2021 Nov 30. PubMed |
| Efficient Generation of P53 Biallelic Mutations in Diannan Miniature Pigs Using RNA-Guided Base Editing. Li H, Cheng W, Chen B, Pu S, Fan N, Zhang X, Jiao D, Shi D, Guo J, Li Z, Qing Y, Jia B, Zhao HY, Wei HJ. Life (Basel). 2021 Dec 17;11(12). pii: life11121417. doi: 10.3390/life11121417. PubMed |
| Interdependent Regulation of Polycystin Expression Influences Starvation-Induced Autophagy and Cell Death. Decuypere JP, Van Giel D, Janssens P, Dong K, Somlo S, Cai Y, Mekahli D, Vennekens R. Int J Mol Sci. 2021 Dec 16;22(24). pii: ijms222413511. doi: 10.3390/ijms222413511. PubMed |
| Knockout of circRNAs by base editing back-splice sites of circularized exons. Gao X, Ma XK, Li X, Li GW, Liu CX, Zhang J, Wang Y, Wei J, Chen J, Chen LL, Yang L. Genome Biol. 2022 Jan 10;23(1):16. doi: 10.1186/s13059-021-02563-0. PubMed |
| Rapid and robust derivation of mesenchymal stem cells from human pluripotent stem cells via temporal induction of neuralized ectoderm. Jin W, He Y, Li T, Long F, Qin X, Yuan Y, Gao G, Shakhawat HM, Liu X, Jin G, Zhou Z. Cell Biosci. 2022 Mar 15;12(1):31. doi: 10.1186/s13578-022-00753-2. PubMed |
| Precise tumor immune rewiring via synthetic CRISPRa circuits gated by concurrent gain/loss of transcription factors. Wang Y, Zhang G, Meng Q, Huang S, Guo P, Leng Q, Sun L, Liu G, Huang X, Liu J. Nat Commun. 2022 Mar 18;13(1):1454. doi: 10.1038/s41467-022-29120-y. PubMed |
| ABCA1 Expression Is Upregulated in an EMT in Breast Cancer Cell Lines via MYC-Mediated De-Repression of Its Proximal Ebox Element. Prijic S, Chang JT. Biomedicines. 2022 Mar 2;10(3). pii: biomedicines10030581. doi: 10.3390/biomedicines10030581. PubMed |
| Highly efficient prime editing by introducing same-sense mutations in pegRNA or stabilizing its structure. Li X, Zhou L, Gao BQ, Li G, Wang X, Wang Y, Wei J, Han W, Wang Z, Li J, Gao R, Zhu J, Xu W, Wu J, Yang B, Sun X, Yang L, Chen J. Nat Commun. 2022 Mar 29;13(1):1669. doi: 10.1038/s41467-022-29339-9. PubMed |
| Prdm12 regulates inhibitory neuron differentiation in mouse embryonal carcinoma cells. Baba A, Suwada T, Muta S, Kuhara S, Tashiro K. Cytotechnology. 2022 Apr;74(2):329-339. doi: 10.1007/s10616-022-00519-1. Epub 2022 Feb 2. PubMed |
| Production of Triple-Gene (GGTA1, B2M and CIITA)-Modified Donor Pigs for Xenotransplantation. Xu K, Yu H, Chen S, Zhang Y, Guo J, Yang C, Jiao D, Nguyen TD, Zhao H, Wang J, Wei T, Li H, Jia B, Jamal MA, Zhao HY, Huang X, Wei HJ. Front Vet Sci. 2022 Apr 28;9:848833. doi: 10.3389/fvets.2022.848833. eCollection 2022. PubMed |
| Efficient Simultaneous Introduction of Premature Stop Codons in Three Tumor Suppressor Genes in PFFs via a Cytosine Base Editor. Jiang H, Jing Q, Yang Q, Qiao C, Liao Y, Liu W, Xing Y. Genes (Basel). 2022 May 7;13(5). pii: genes13050835. doi: 10.3390/genes13050835. PubMed |
| A TET1-PSPC1-Neat1 molecular axis modulates PRC2 functions in controlling stem cell bivalency. Huang X, Bashkenova N, Hong Y, Lyu C, Guallar D, Hu Z, Malik V, Li D, Wang H, Shen X, Zhou H, Wang J. Cell Rep. 2022 Jun 7;39(10):110928. doi: 10.1016/j.celrep.2022.110928. PubMed |
| Cloning and base editing of GFP transgenic rhesus monkey and off-target analysis. Kang Y, Dai S, Zeng Y, Wang F, Yang P, Yang Z, Pu Y, Li Z, Chen X, Tian B, Si W, Ji W, Niu Y. Sci Adv. 2022 Jul 22;8(29):eabo3123. doi: 10.1126/sciadv.abo3123. Epub 2022 Jul 22. PubMed |
| Homeodomain-interacting protein kinase HIPK4 regulates phosphorylation of manchette protein RIMBP3 during spermiogenesis. Liu X, Zang C, Wu Y, Meng R, Chen Y, Jiang T, Wang C, Yang X, Guo Y, Situ C, Hu Z, Zhang J, Guo X. J Biol Chem. 2022 Sep;298(9):102327. doi: 10.1016/j.jbc.2022.102327. Epub 2022 Aug 2. PubMed |
| Characterization and perturbation of CTCF-mediated chromatin interactions for enhancing myogenic transdifferentiation. Ren R, Fan Y, Peng Z, Wang S, Jiang Y, Fu L, Cao J, Zhao S, Wang H. Cell Rep. 2022 Aug 16;40(7):111206. doi: 10.1016/j.celrep.2022.111206. PubMed |
| Knockouts of TUT7 and 3'hExo show that they cooperate in histone mRNA maintenance and degradation. Holmquist CE, He W, Meganck RM, Marzluff WF. RNA. 2022 Nov;28(11):1519-1533. doi: 10.1261/rna.079233.122. Epub 2022 Aug 30. PubMed |
| XBP1 Activation Reduces Severity of Polycystic Kidney Disease due to a Nontruncating Polycystin-1 Mutation in Mice. Krappitz M, Bhardwaj R, Dong K, Staudner T, Yilmaz DE, Pioppini C, Westergerling P, Ruemmele D, Hollmann T, Nguyen TA, Cai Y, Gallagher AR, Somlo S, Fedeles S. J Am Soc Nephrol. 2023 Jan 1;34(1):110-121. doi: 10.1681/ASN.2021091180. Epub 2022 Oct 21. PubMed |
| Automated high-throughput genome editing platform with an AI learning in situ prediction model. Li S, An J, Li Y, Zhu X, Zhao D, Wang L, Sun Y, Yang Y, Bi C, Zhang X, Wang M. Nat Commun. 2022 Nov 30;13(1):7386. doi: 10.1038/s41467-022-35056-0. PubMed |
| Microhomology-mediated endjoining repair mechanism enables rapid and effective indel generations in stem cells. Yadav N, Thelma BK. J Biosci. 2022;47:72. PubMed |
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