PurposeEndogenous gene activation with the SunTag CRISPR activation system
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||121119||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse), Synthetic
- Promoter Tet-ON
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
This PiggyBac vector encodes for the two components of the SunTag system for gene activation. Both transgenes are driven by TetON promoters which also drive resistance to Hygromycin under Doxycycline resistance.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PB-TetON-dual-SunTag-Hygro was a gift from Pablo Navarro (Addgene plasmid # 121119 ; http://n2t.net/addgene:121119 ; RRID:Addgene_121119)
For your References section:The molecular logic of Nanog-induced self-renewal in mouse embryonic stem cells. Heurtier V, Owens N, Gonzalez I, Mueller F, Proux C, Mornico D, Clerc P, Dubois A, Navarro P. Nat Commun. 2019 Mar 7;10(1):1109. doi: 10.1038/s41467-019-09041-z. 10.1038/s41467-019-09041-z PubMed 30846691