|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20910||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6262
Vector typeMammalian Expression ; piggyBac
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)754
- Cloning method Restriction Enzyme
- 5′ cloning site attB1 (not destroyed)
- 3′ cloning site attB2 (not destroyed)
- 5′ sequencing primer M13 R, T3
- 3′ sequencing primer M13F, T7 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bypiggyBac terminal repeats obtained from Pentao Liu at Sanger, CAG-rtTA Advanced obtained from Clontech.
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PB-CA-rtTA Adv was a gift from Andras Nagy (Addgene plasmid # 20910 ; http://n2t.net/addgene:20910 ; RRID:Addgene_20910)
For your References section:piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Woltjen K, Michael IP, Mohseni P, Desai R, Mileikovsky M, Hämäläinen R, Cowling R, Wang W, Liu P, Gertsenstein M, Kaji K, Sung HK, Nagy A. Nature. 2009 Mar 1. ():. 10.1038/nature07863 PubMed 19252478