PurposepiggyBac vector with tet inducible mKLF4
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20906||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9186
Vector typeMammalian Expression ; piggyBac
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)1479
Entrez GeneKlf4 (a.k.a. EZF, Gklf, Zie)
/ Fusion Protein
- IRES-bGeo (C terminal on backbone)
- Cloning method Gateway Cloning
- 5′ cloning site attB1 (not destroyed)
- 3′ cloning site attB2 (not destroyed)
- 5′ sequencing primer M13 F, T7
- 3′ sequencing primer M13 R, T3 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byKLF4 gateway cloned from Yamanaka Plasmid 13370: pMXs-Klf4, piggyBac terminal repeats obtained from Pentao Liu at Sanger.
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PB-TET-mKLF4 was a gift from Andras Nagy (Addgene plasmid # 20906 ; http://n2t.net/addgene:20906 ; RRID:Addgene_20906)
For your References section:piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Woltjen K, Michael IP, Mohseni P, Desai R, Mileikovsky M, Hämäläinen R, Cowling R, Wang W, Liu P, Gertsenstein M, Kaji K, Sung HK, Nagy A. Nature. 2009 Mar 1. ():. 10.1038/nature07863 PubMed 19252478