Self-delivering, chemically modified CRISPR RNAs for AAV co-delivery and genome editing in vivo.
Zhang H, Kelly K, Lee J, Echeverria D, Cooper D, Panwala R, Amrani N, Chen Z, Gaston N, Wagh A, Newby GA, Xie J, Liu DR, Gao G, Wolfe SA, Khvorova A, Watts JK, Sontheimer EJ
Nucleic Acids Res. 2023 Nov 30:gkad1125. doi: 10.1093/nar/gkad1125. PubMed Article
Zhang H, Kelly K, Lee J, Echeverria D, Cooper D, Panwala R, Amrani N, Chen Z, Gaston N, Wagh A, Newby GA, Xie J, Liu DR, Gao G, Wolfe SA, Khvorova A, Watts JK, Sontheimer EJ
Nucleic Acids Res. 2023 Nov 30:gkad1125. doi: 10.1093/nar/gkad1125. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 211815 | pEJS-HZ03-pLenti-U1a-mSpCas9-U6-tracrRNA-PuroR | lentiviral vector expressing SpCas9 and tracrRNA for stable cell line establishment |
| 211816 | pEJS-HZ08-pAAVsc.U6-tracrRNA | AAV vector expressing tracrRNA under U6 promoter |
| 211817 | pEJS-HZ96 AAV-SpABE8e-N-terminus_tracrRNA | AAV vector expressing N-terminal of SpCas9-ABE8e and tracrRNA |
| 211818 | pEJS-HZ100 AAV-SpABE8e-C_terminus-tracrRNA | AAV vector expressing C-terminus of SpCas9-ABE8e with tracrRNA |
| 211819 | pEJS1553 pLenti-U1a-SpCas9-miniU6-tracrRNA | lentiviral vector expressing SpCas9 and tracrRNA for stable cell line extablishment |
| 211820 | pEJS1983 Lenti-dead_GFP_ABE reporter-BlastR | Lentiviral vector expressing a dead GFP adenine base editing reporter |
| 211821 | pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro | PiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishment |