166,778 results

1. pEf1a-hifiDn29-dCas9-P2A-GFP

   Plasmid

   #247158

   Purpose

   Mammalian expression of a human codon optimized engineered hifiDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3

   Depositor

   Patrick Hsu

   Article

   Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

   Insert

   hifiDn29-dCas9-P2A-GFP
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…

   Promoter

   Ef1a

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW-Cas9-2A-EGFP

   Plasmid

   #167928

   Purpose

   Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Insert

   T2A-EGFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   tTRE, hPGK

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSOUP

   Plasmid

   #165419

   Purpose

   The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

   Depositor

   Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

   Article

   Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.

   Insert

   Rep A
   

   Use

   Helper plasmid required for replication for all p…

   Available Since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP- SEC61B

   Plasmid

   #121159

   Purpose

   Mammalian expression of GFP tagged SEC61B, used as a general ER marker

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Insert

   SEC61B (SEC61B Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMKO.1 GFP

   Plasmid

   #10676

   Purpose

   Retroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.

   Depositor

   William Hahn

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti CMV GFP Hygro (656-4)

   Plasmid

   #17446

   Purpose

   3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Lentiviral Prep

   Insert

   GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti PGK GFP Puro (w509-5)

   Plasmid

   #19070

   Purpose

   3rd gen lentiviral eGFP expression vector, PGK promoter, Puro 

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   enhanced green fluorescent protein
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJAM1.19_PB-CIT-PGK-Blast

   Plasmid

   #205564

   Purpose

   Express human CIT

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   CIT (CIT Human)
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAXY0002

   Plasmid

   #179835

   Purpose

   binary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoter

   Depositor

   Xiaohan Yang

   Article

   Yuan et al Hortic Res. 2021 Nov 1;8(1):234. doi: 10

   Type

   Empty backbone

   Use

   Binary vector for plant transformation

   Expression

   Plant

   Available Since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJAM1.20_PB-NEDD4L_deltaC2-PGK-Blast

    Plasmid

    #205565

    Purpose

    Express human NEDD4L (delta C2 isoform)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    NEDD4L (NEDD4L Human)
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    delta C2 isoform

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCXWB-EBNA1

    Plasmid

    #37624

    Purpose

    Non-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmids

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    EBNA-1 (EBNA-1 human herpesvirus 4)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

    Plasmid

    #42335

    Purpose

    A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Has Service

    Cloning Grade DNA

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG CMV Empty Puro

    Plasmid

    #237881

    Purpose

    Empty mammalian expression plasmid for cloning untagged proteins

    Depositor

    Meghan Rego, Addgene Research Program

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 12, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pLV-EF1a-IRES-Hygro

    Plasmid

    #85134

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-LSSmGFP

    Plasmid

    #186543

    Purpose

    LSSmGFP fluorescent protein (ex/em = 400/510 nm): cytosolic expression in mammalian cells

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP
    

    Expression

    Mammalian

    Mutation

    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. rAAV2-retro helper

    Plasmid

    #81070

    Purpose

    retrograde AAV helper vector, carrying rep and modified capsid gene

    Depositor

    Alla Karpova, David Schaffer

    Article

    Tervo et al Neuron. 2016 Oct 6. pii: S0896-6273(16)3

    Inserts

    rep
    rAAV2-retro cap
    

    Use

    AAV and Unspecified; Helper vector

    Mutation

    Pro2Ala, possibly Asp613Gly and changes made to A…

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1 mCherry

    Plasmid

    #128073

    Purpose

    puro exchanged to mCherry

    Depositor

    Oskar Laur

    Insert

    mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    no

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAVS1-TRE3G-EGFP

    Plasmid

    #52343

    Purpose

    AAVS1 donor vector for inducible gene expression

    Depositor

    Su-Chun Zhang

    Article

    Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

    Insert

    EGFP
    

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. EZ-Tet-pLKO-Hygro

    Plasmid

    #85972

    Purpose

    Tet/Dox inducible shRNA lentivirus with hygromycin selection

    Depositor

    Cindy Miranti

    Article

    Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Promoter

    H1 / TetO

    Available Since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEB1-mGFPmut2

    Plasmid

    #103980

    Purpose

    Plasmid encoding mGFPmut2

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mGFPmut2
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S65A, V68L, …

    Promoter

    proC

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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