Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

pCRY2PHR-mCherryN1 Citations (32)

Originally described in: Rapid blue-light-mediated induction of protein interactions in living cells.
Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL Nat Methods. 2010 Dec;7(12):973-5. doi: 10.1038/nmeth.1524 PubMed Journal

Articles Citing pCRY2PHR-mCherryN1

Articles
Quantitative real-time kinetics of optogenetic proteins CRY2 and CIB1/N using single-molecule tools. Cui Y, Choudhury SR, Irudayaraj J. Anal Biochem. 2014 Aug 1;458:58-60. doi: 10.1016/j.ab.2014.04.023. Epub 2014 Apr 26. PubMed
Subcellular optogenetic inhibition of G proteins generates signaling gradients and cell migration. O'Neill PR, Gautam N. Mol Biol Cell. 2014 Aug 1;25(15):2305-14. doi: 10.1091/mbc.E14-04-0870. Epub 2014 Jun 11. PubMed

Associated Plasmids
Tools for controlling protein interactions using light. Tucker CL, Vrana JD, Kennedy MJ. Curr Protoc Cell Biol. 2014 Sep 2;64:17.16.1-17.16.20. doi: 10.1002/0471143030.cb1716s64. PubMed
Examining estrogen regulation of cancer stem cells through multicolor lineage tracing. Zhang Y, Eades G, Zhou Q. Methods Mol Biol. 2014;1204:35-44. doi: 10.1007/978-1-4939-1346-6_4. PubMed
Optogenetic regulation of site-specific subtelomeric DNAmethylation. Choudhury SR, Cui Y, Narayanan A, Gilley DP, Huda N, Lo CL, Zhou FC, Yernool D, Irudayaraj J. Oncotarget. 2016 Jul 4. doi: 10.18632/oncotarget.10394. PubMed

Associated Plasmids
Complex transcriptional modulation with orthogonal and inducible dCas9 regulators. Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS. Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. PubMed

Associated Plasmids
Transcription activator-like effector-mediated regulation of gene expression based on the inducible packaging and delivery via designed extracellular vesicles. Lainscek D, Lebar T, Jerala R. Biochem Biophys Res Commun. 2017 Feb 26;484(1):15-20. doi: 10.1016/j.bbrc.2017.01.090. Epub 2017 Jan 19. PubMed
Optogenetic Control of Synaptic Composition and Function. Sinnen BL, Bowen AB, Forte JS, Hiester BG, Crosby KC, Gibson ES, Dell'Acqua ML, Kennedy MJ. Neuron. 2017 Feb 8;93(3):646-660.e5. doi: 10.1016/j.neuron.2016.12.037. Epub 2017 Jan 26. PubMed

Associated Plasmids
Optogenetic inhibition of apical constriction during Drosophila embryonic development. Guglielmi G, De Renzis S. Methods Cell Biol. 2017;139:167-186. doi: 10.1016/bs.mcb.2016.10.007. Epub 2016 Nov 23. PubMed
Temporally precise labeling and control of neuromodulatory circuits in the mammalian brain. Lee D, Creed M, Jung K, Stefanelli T, Wendler DJ, Oh WC, Mignocchi NL, Luscher C, Kwon HB. Nat Methods. 2017 May;14(5):495-503. doi: 10.1038/nmeth.4234. Epub 2017 Apr 3. PubMed

Associated Plasmids
Decoding temporal interpretation of the morphogen Bicoid in the early Drosophila embryo. Huang A, Amourda C, Zhang S, Tolwinski NS, Saunders TE. Elife. 2017 Jul 10;6. doi: 10.7554/eLife.26258. PubMed
Design and Profiling of a Subcellular Targeted Optogenetic cAMP-Dependent Protein Kinase. O'Banion CP, Priestman MA, Hughes RM, Herring LE, Capuzzi SJ, Lawrence DS. Cell Chem Biol. 2017 Oct 25. pii: S2451-9456(17)30355-0. doi: 10.1016/j.chembiol.2017.09.011. PubMed

Associated Plasmids
Real-time observation of light-controlled transcription in living cells. Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K. J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. Epub 2017 Nov 9. PubMed

Associated Plasmids
Spatiotemporal control of zebrafish (Danio rerio) gene expression using a light-activated CRISPR activation system. Putri RR, Chen L. Gene. 2018 Nov 30;677:273-279. doi: 10.1016/j.gene.2018.07.077. Epub 2018 Aug 1. PubMed
A Photoactivatable Botulinum Neurotoxin for Inducible Control of Neurotransmission. Liu Q, Sinnen BL, Boxer EE, Schneider MW, Grybko MJ, Buchta WC, Gibson ES, Wysoczynski CL, Ford CP, Gottschalk A, Aoto J, Tucker CL, Kennedy MJ. Neuron. 2019 Jan 15. pii: S0896-6273(19)30003-0. doi: 10.1016/j.neuron.2019.01.002. PubMed

Associated Plasmids
Continued Activity of the Pioneer Factor Zelda Is Required to Drive Zygotic Genome Activation. McDaniel SL, Gibson TJ, Schulz KN, Fernandez Garcia M, Nevil M, Jain SU, Lewis PW, Zaret KS, Harrison MM. Mol Cell. 2019 Apr 4;74(1):185-195.e4. doi: 10.1016/j.molcel.2019.01.014. Epub 2019 Feb 20. PubMed
RNA Binding Antagonizes Neurotoxic Phase Transitions of TDP-43. Mann JR, Gleixner AM, Mauna JC, Gomes E, DeChellis-Marks MR, Needham PG, Copley KE, Hurtle B, Portz B, Pyles NJ, Guo L, Calder CB, Wills ZP, Pandey UB, Kofler JK, Brodsky JL, Thathiah A, Shorter J, Donnelly CJ. Neuron. 2019 Feb 27. pii: S0896-6273(19)30075-3. doi: 10.1016/j.neuron.2019.01.048. PubMed
Chronic optogenetic induction of stress granules is cytotoxic and reveals the evolution of ALS-FTD pathology. Zhang P, Fan B, Yang P, Temirov J, Messing J, Kim HJ, Taylor JP. Elife. 2019 Mar 20;8. pii: 39578. doi: 10.7554/eLife.39578. PubMed
Optogenetics reveals Cdc42 local activation by scaffold-mediated positive feedback and Ras GTPase. Lamas I, Merlini L, Vjestica A, Vincenzetti V, Martin SG. PLoS Biol. 2020 Jan 24;18(1):e3000600. doi: 10.1371/journal.pbio.3000600. eCollection 2020 Jan. PubMed
Perfluorooctanoic acid (PFOA) exposure inhibits DNA methyltransferase activities and alters constitutive heterochromatin organization. Liu W, Irudayaraj J. Food Chem Toxicol. 2020 Jul;141:111358. doi: 10.1016/j.fct.2020.111358. Epub 2020 Apr 18. PubMed
Multiple Sclerosis-Associated hnRNPA1 Mutations Alter hnRNPA1 Dynamics and Influence Stress Granule Formation. Clarke JWE, Thibault PA, Salapa HE, Kim DE, Hutchinson C, Levin MC. Int J Mol Sci. 2021 Mar 12;22(6). pii: ijms22062909. doi: 10.3390/ijms22062909. PubMed
Liquid-like protein interactions catalyse assembly of endocytic vesicles. Day KJ, Kago G, Wang L, Richter JB, Hayden CC, Lafer EM, Stachowiak JC. Nat Cell Biol. 2021 Apr;23(4):366-376. doi: 10.1038/s41556-021-00646-5. Epub 2021 Apr 5. PubMed
Optogenetics-based localization of talin to the plasma membrane promotes activation of beta3 integrins. Liao Z, Gingras AR, Lagarrigue F, Ginsberg MH, Shattil SJ. J Biol Chem. 2021 Jan-Jun;296:100675. doi: 10.1016/j.jbc.2021.100675. Epub 2021 Apr 16. PubMed
Using optogenetics to link myosin patterns to contractile cell behaviors during convergent extension. Herrera-Perez RM, Cupo C, Allan C, Lin A, Kasza KE. Biophys J. 2021 Jul 20. pii: S0006-3495(21)00597-X. doi: 10.1016/j.bpj.2021.06.041. PubMed
Multivalent electrostatic pi-cation interaction between synaptophysin and synapsin is responsible for the coacervation. Kim G, Lee SE, Jeong S, Lee J, Park D, Chang S. Mol Brain. 2021 Sep 8;14(1):137. doi: 10.1186/s13041-021-00846-y. PubMed
Light-activated mitochondrial fission through optogenetic control of mitochondria-lysosome contacts. Qiu K, Zou W, Fang H, Hao M, Mehta K, Tian Z, Guan JL, Zhang K, Huang T, Diao J. Nat Commun. 2022 Jul 25;13(1):4303. doi: 10.1038/s41467-022-31970-5. PubMed
CRY-BARs: Versatile light-gated molecular tools for the remodeling of membrane architectures. Wurz AI, Bunner WP, Szatmari EM, Hughes RM. J Biol Chem. 2022 Aug 18;298(10):102388. doi: 10.1016/j.jbc.2022.102388. PubMed

Associated Plasmids
Cell clusters adopt a collective amoeboid mode of migration in confined nonadhesive environments. Pages DL, Dornier E, de Seze J, Gontran E, Maitra A, Maciejewski A, Wang L, Luan R, Cartry J, Canet-Jourdan C, Raingeaud J, Lemahieu G, Lebel M, Ducreux M, Gelli M, Scoazec JY, Coppey M, Voituriez R, Piel M, Jaulin F. Sci Adv. 2022 Sep 30;8(39):eabp8416. doi: 10.1126/sciadv.abp8416. Epub 2022 Sep 30. PubMed
Spatiotemporal dynamics of membrane surface charge regulates cell polarity and migration. Banerjee T, Biswas D, Pal DS, Miao Y, Iglesias PA, Devreotes PN. Nat Cell Biol. 2022 Oct;24(10):1499-1515. doi: 10.1038/s41556-022-00997-7. Epub 2022 Oct 6. PubMed

Associated Plasmids
WNK kinases sense molecular crowding and rescue cell volume via phase separation. Boyd-Shiwarski CR, Shiwarski DJ, Griffiths SE, Beacham RT, Norrell L, Morrison DE, Wang J, Mann J, Tennant W, Anderson EN, Franks J, Calderon M, Connolly KA, Cheema MU, Weaver CJ, Nkashama LJ, Weckerly CC, Querry KE, Pandey UB, Donnelly CJ, Sun D, Rodan AR, Subramanya AR. Cell. 2022 Nov 23;185(24):4488-4506.e20. doi: 10.1016/j.cell.2022.09.042. Epub 2022 Oct 31. PubMed
Optogenetic modulation of guanine nucleotide exchange factors of Ras superfamily proteins directly controls cell shape and movement. Pal DS, Lin Y, Zhan H, Banerjee T, Kuhn J, Providence S, Devreotes PN. Front Cell Dev Biol. 2023 Jul 10;11:1195806. doi: 10.3389/fcell.2023.1195806. eCollection 2023. PubMed
Ubiquitin-driven protein condensation promotes clathrin-mediated endocytosis. Yuan F, Gollapudi S, Day KJ, Ashby G, Sangani A, Malady BT, Wang L, Lafer EM, Huibregtse JM, Stachowiak JC. bioRxiv [Preprint]. 2023 Aug 22:2023.08.21.554139. doi: 10.1101/2023.08.21.554139. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.