ECON
(Plasmid #82553)

• Purpose: Epigenetic effector construct for targeted methylation
• Depositing Lab: Joseph Irudayaraj
• Publication: Choudhury et al Oncotarget. 2016 Jul 4. doi: 10.18632/oncotarget.10394.

Backbone

• Vector backbone: pHTN HaloTag(R) CMV-neo
• Backbone manufacturer: Promega
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: DNMT3A
• Species: H. sapiens (human)
• Entrez Gene: DNMT3A (a.k.a. DNMT3A2, HESJAS, M.HsaIIIA, TBRS)
• Promoter: CMV
• Tag / Fusion Protein:
  • HALO (N terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: unknown

Gene/Insert 2

• Gene/Insert name: CRY2
• Species: A. thaliana (mustard weed)
• Mutation: N-terminal photolyase homology region domain of CRY2 only
• Entrez Gene: CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: DNMT3A was obtained from #35521 from A. Riggs Laboratory. CRY2PHR-mCherry was amplified from the plasmid #26866 from C. Tucker Laboratory.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry