PurposeLocalizes CRY2 to excitatory synapses
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||89442||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesR. norvegicus (rat), A. thaliana (mustard weed)
MutationContains Full length CRY2
- Promoter CAG
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
strong promoter-high expression levels lead to aggregation. Use~150ng plasmid for one well of a 12 well plate
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CRY2-GFP-homer1c was a gift from Matthew Kennedy (Addgene plasmid # 89442 ; http://n2t.net/addgene:89442 ; RRID:Addgene_89442)
For your References section:Optogenetic Control of Synaptic Composition and Function. Sinnen BL, Bowen AB, Forte JS, Hiester BG, Crosby KC, Gibson ES, Dell'Acqua ML, Kennedy MJ. Neuron. 2017 Feb 8;93(3):646-660.e5. doi: 10.1016/j.neuron.2016.12.037. Epub 2017 Jan 26. 10.1016/j.neuron.2016.12.037 PubMed 28132827