pAAV hSyn mCh-IRES-BoNT/B(1-146)-iLID
PurposeAAV plasmid with human synapsin promoter driving mCh and BoNT/B amino acids 1-146 fused to iLID(V416I), separated with an IRES element. Co-express with SSPB-BoNT(147-441, Y365A) for sPA-BoNT
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||122982||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
Currently unavailable outside the U.S.
This material is available to academics and nonprofits only.
Vector backbonepAAV hSyn
- Backbone size w/o insert (bp) 4500
- Total vector size (bp) 6800
Vector typeMammalian Expression, AAV
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Insert Size (bp)2300
MutationiLID contains long lived V416I mutation
- Promoter human synapsin
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer actcagcgctgcctcagt
- 3′ sequencing primer ccacatagcgtaaaaggagcaac (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byiLID was obtained from Dr. Brian Kuhlman's lab (University of North Carolina, Chapel Hill) BoNT/B was a gift from Dr Thomas Binz (Hannover Medical School)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV hSyn mCh-IRES-BoNT/B(1-146)-iLID was a gift from Matthew Kennedy (Addgene plasmid # 122982 ; http://n2t.net/addgene:122982 ; RRID:Addgene_122982)
For your References section:A Photoactivatable Botulinum Neurotoxin for Inducible Control of Neurotransmission. Liu Q, Sinnen BL, Boxer EE, Schneider MW, Grybko MJ, Buchta WC, Gibson ES, Wysoczynski CL, Ford CP, Gottschalk A, Aoto J, Tucker CL, Kennedy MJ. Neuron. 2019 Jan 15. pii: S0896-6273(19)30003-0. doi: 10.1016/j.neuron.2019.01.002. 10.1016/j.neuron.2019.01.002 PubMed 30704911