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Real-time observation of light-controlled transcription in living cells.
Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K
J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. Epub 2017 Nov 9.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
103797pCIBN-tagBFP-TetRBLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light
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103798pCIBN-EYFP-TetRBLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light
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103799pCIBN-tagBFP-hLaminB1BLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue light
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103800pCIBN-EYFP-hLaminB1BLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue light
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103801pCIBN-tagBFP-hTRF1BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light
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103802pCIBN-EYFP-hTRF1BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light
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103803pCIBN-tagBFP-hTRF2BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light
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103804pCIBN-EYFP-hTRF2BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light
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103805pCIBN-tagBFP-hNCLBLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light
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103806pCIBN-EYFP-hNCLBLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light
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103807pCIBN-tagBFP-hPMLIIIBLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue light
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103808pCIBN-EYFP-hPMLIIIBLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue light
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103809pCIBN-TetR-tagRFP-T'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment
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103810pCIBN-LacI-tagRFP-T'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment
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103811pCIBN-hTRF1-tagRFP-TBLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment
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103812pCIBN-hTRF2-tagRFP-TBLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment
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103813pCIBN-TetRBLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light
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103814pCIBN-LacIBLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light
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103815pCIBN-EYFPSoluble BLInCR construct that can interact with PHR constructs upon illumination with blue light
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103816pCIBN-mCherrySoluble BLInCR construct that can interact with PHR constructs upon illumination with blue light
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103817pPHR-EYFPSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination
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103818pPHR-iRFP713Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination
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103819pPHR-mCherry-NLSSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination
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103820pPHR-EYFP-NLSSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination
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103821pPHR-mCherry-NLS-VP16Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination
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103822pPHR-EYFP-NLS-VP16Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination
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103823pPHR-iRFP713-NLS-VP16Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination
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103824pPHR-mCherry-hPMLIIISoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination
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103825pPHR-EYFP-hPMLIIISoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination
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103826pPHR-EYFP-hGCN5Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination
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103827pPHR-EYFP-hGCN5-NLSSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination
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103828pPHR-EYFP-hGCN5mutSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination
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103829pPHR-EYFP-hHP1βSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination
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103830pPHR-mCherry-hHP1βSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination
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103831ptagRFP-MS2coatProteinLive cell reader for MS2 loop RNA production
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103832pmCherry-MS2coatProteinLive cell reader for MS2 loop RNA production
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103833pmKate2-MS2coatProteinLive cell reader for MS2 loop RNA production
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103834pEGFP-TetR-NLS-VP16Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays
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103835ptagBFP-TetR-NLS-VP16Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays
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103836pEGFP-LacI-NLS-VP16Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays
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103837ptagBFP-LacI-NLS-VP16Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays
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103838pNLS-TetR-GFPFlourescent marker for tetO arrays
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103839ptagBFP-LacIFlourescent marker for lacO arrays
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103840pCFP-LacI-NLSFlourescent marker for lacO arrays
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