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pCI-His-hUbi
(Plasmid #31815)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31815 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCI
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ubiquitin
  • Alt name
    UBB
  • Species
    H. sapiens (human)
  • Entrez Gene
    UBB (a.k.a. HEL-S-50)
  • Promoter CMV
  • Tag / Fusion Protein
    • HIS (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer EBV reverse
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Ubiquitin was PCR amplified from pcDNA3-HAUbiquitin (Addgene #18712) for cloning into this vector. As noted on the plasmid page for #18712: "The HA-Ubiquitin insert is from the UBB gene. The reason for the small size is because it contains a single ubiquitin coding sequence (full length UBB has three repeating ubiquitin coding sequences)."

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-His-hUbi was a gift from Astar Winoto (Addgene plasmid # 31815 ; http://n2t.net/addgene:31815 ; RRID:Addgene_31815)
  • For your References section:

    Fas-associated death domain (FADD) and the E3 ubiquitin-protein ligase TRIM21 interact to negatively regulate virus-induced interferon production. Young JA, Sermwittayawong D, Kim HJ, Nandu S, An N, Erdjument-Bromage H, Tempst P, Coscoy L, Winoto A. J Biol Chem. 2011 Feb 25;286(8):6521-31. Epub 2010 Dec 23. 10.1074/jbc.M110.172288 PubMed 21183682