|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31815||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneUBB (a.k.a. HEL-S-50)
- Promoter CMV
/ Fusion Protein
- HIS (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer CMV forward
- 3′ sequencing primer EBV reverse (Common Sequencing Primers)
Ubiquitin was PCR amplified from pcDNA3-HAUbiquitin (Addgene #18712) for cloning into this vector. As noted on the plasmid page for #18712: "The HA-Ubiquitin insert is from the UBB gene. The reason for the small size is because it contains a single ubiquitin coding sequence (full length UBB has three repeating ubiquitin coding sequences)."
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCI-His-hUbi was a gift from Astar Winoto (Addgene plasmid # 31815 ; http://n2t.net/addgene:31815 ; RRID:Addgene_31815)
For your References section:Fas-associated death domain (FADD) and the E3 ubiquitin-protein ligase TRIM21 interact to negatively regulate virus-induced interferon production. Young JA, Sermwittayawong D, Kim HJ, Nandu S, An N, Erdjument-Bromage H, Tempst P, Coscoy L, Winoto A. J Biol Chem. 2011 Feb 25;286(8):6521-31. Epub 2010 Dec 23. 10.1074/jbc.M110.172288 PubMed 21183682