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Inducible Caspex expression
(Plasmid #97421)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 97421 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 97421-D50 50 μg of DNA in Tris buffer $495
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DNA 97421-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    PB-TetON
  • Backbone manufacturer
    George Church lab
  • Backbone size w/o insert (bp) 7501
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Caspex
  • Alt name
    CATG
  • Alt name
    Genomic Locus Proteomics
  • Species
    S. pyogenes, Soybean
  • Mutation
    dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, A134P
  • Promoter TRE3G
  • Tags / Fusion Proteins
    • 3xFLAG (N terminal on insert)
    • V5 (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer ggcctccacggccactagt
  • 3′ sequencing primer EF1a-rev
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    T2A-Gfp
  • Species
    Jellyfish

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are ITRs for piggybac transposase insertion but no transposase is in the plasmid itself.

bioRxiv preprint: https://doi.org/10.1101/159517 Posted July 4, 2017.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Inducible Caspex expression was a gift from Steven Carr & Samuel Myers (Addgene plasmid # 97421 ; http://n2t.net/addgene:97421 ; RRID:Addgene_97421)

  • For your References section:

    Discovery of proteins associated with a predefined genomic locus via dCas9-APEX-mediated proximity labeling. Myers SA, Wright J, Peckner R, Kalish BT, Zhang F, Carr SA. Nat Methods. 2018 May 7. pii: 10.1038/s41592-018-0007-1. doi: 10.1038/s41592-018-0007-1. 10.1038/s41592-018-0007-1 PubMed 29735997
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