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Lenti-miniTurbo-NLS-Blast

Plasmid

#232347

Purpose

Lentiviral control vector expressing miniTurboNLS without dCas9

Depositor

Ali Shilatifard

Article

Cenik et al Mol Cell. 2024 Dec 19;84(24):4929-4944.e

Insert

miniTurbo

Use

Lentiviral

Tags

3xHA and 3xSV40 NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1-TALEN-L

Plasmid

#59025

Purpose

TALEN designed to target the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

AAVS1-TALEN-L

Use

AAV and TALEN

Tags

3xFlag and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2014

Availability

Academic Institutions and Nonprofits only

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AAVS1-TALEN-R

Plasmid

#59026

Purpose

TALEN designed to target the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

AAVS1-TALEN-R

Use

AAV and TALEN

Tags

3xFlag and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2014

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-N1

Plasmid

#54843

Purpose

Localization: N1 Cloning Vector, Excitation: 434, Emission: 474

Depositor

Michael Davidson, Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Type

Empty backbone

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pZT-C13-L1

Plasmid

#62196

Purpose

Chr.13/CLYBL TALEN expression vector

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

Left CLYBL TALEN

Use

TALEN

Tags

Expression

Mutation

Promoter

CMV

Available since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19

Plasmid

#50005

Purpose

pUC cloning vector

Depositor

Joachim Messing

Article

Norrander et al Gene. 1983 Dec;26(1):101-6.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

lac

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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EGFPC1-huNFATc1EE-WT

Plasmid

#24219

Depositor

Jerry Crabtree

Article

Beals et al Genes Dev. 1997 Apr 1. 11(7):824-34.

Insert

NFATc1 (NFATC1 Human)

Use

Tags

EE and GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pHIV-EF1a-2A-Ovalbumin(dN)-ZsGreen

Plasmid

#231986

Purpose

Empty backbone for expression of Ovalbumin with Bi-cistronic expression ZsGreen

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

OVA-2A-ZsGreen

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau E14 P301L

Plasmid

#46909

Purpose

expresses EGFP tagged Tau E14 P301L in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau E14 P301L (MAPT Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

P301L AND all 14 S/P or T/P amino acid residues (…

Promoter

CMV

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau AP P301L

Plasmid

#46906

Purpose

expresses EGFP tagged Tau AP P301L in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau AP P301L (MAPT Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

P301L AND all 14 S/P or T/P amino acid residues (…

Promoter

CMV

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:ECFP

Plasmid

#158654

Purpose

making transgenics expressing a QUAS driven YFP and NTR 2.0

Depositor

Jeff Mumm

Article

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:eCFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Tags

Expression

Mutation

F70A/F108Y

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pME-QF

Plasmid

#61375

Purpose

Middle Gateway Entry Vector Containing QF

Depositor

Marnie Halpern

Article

Subedi et al Methods. 2014 Apr 1;66(3):433-40. doi: 1

Insert

QF

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pSCALPS-FLAG-HA-mmuMettl3 APPA

Plasmid

#207124

Purpose

Lentiviral plasmid to express mouse Mettl3 APPA mutant

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

Mettl3 (Mettl3 Mouse)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mutation

D395A and W398A

Promoter

SSFV

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-CMV-bArrestin2-TEV

Plasmid

#107245

Purpose

Vector for preparation of stable cell lines expressing Barrestin2-TEV fusion protein in lieu of the HTLA cell line.

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

ARRB2

Use

Tags

TEV(NIA), tobacco etch virus NIA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-GG-acceptor

Plasmid

#132777

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

Exchangeable cassette

Use

Tags

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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35S:RUBY

Plasmid

#160908

Purpose

RUBY under the control of CaMV 35S promoter/marker for transformation

Depositor

Yunde Zhao

Article

He et al Hortic Res. 2020 Sep 19;7:152. doi: 10.1

Insert

RUBY and CaMV 35S promoter

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

Nov. 5, 2020

Availability

Academic Institutions and Nonprofits only

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His6-TEV-CRBN-midi

Plasmid

#215330

Purpose

CRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader design

Depositor

Alessio Ciulli

Article

Kroupova et al Nat Commun. 2024 Oct 15;15(1):8885. doi:

Insert

CRBN (CRBN Human)

Use

Tags

6xHis and TEV

Expression

Bacterial

Mutation

contains amino acids 41-187 followed by a Gly-Ser…

Promoter

lacI

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-ATLASsnCre

Plasmid

#232351

Purpose

Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons, Cre payload. Must use with AAV8-DIO-Reporter viruses infected in postsynaptic cells.

Depositor

Don Arnold

Article

Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

Insert

ATLASsnCre

Use

AAV

Tags

ALFA-Tag

Expression

Mutation

Promoter

hSyn

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-iGluSnFR4s-NGR-WPRE

Plasmid

#234437

Purpose

AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

Depositor

GENIE Project

Article

Aggarwal et al bioRxiv 2025.03.20.643984

Insert

iGluSnFR4s-NGR

Use

AAV

Tags

IgK chain and Myc epi tag-NGR GPI anchor

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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TurboID-His6_pET21a

Plasmid

#107177

Purpose

expresses His6-tagged TurboID in bacteria

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Use

Tags

Expression

Bacterial

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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